Growth hormone-releasing factor (GRF) stimulates PGE2 production in rat anterior pituitary. Evidence for a PGE2 involvement in GRF-induced GH release

Biochemical and Biophysical Research Communications
V FafeurF Dray

Abstract

Rat anterior pituitaries were incubated over a 3-h period. Both PGE2 and GH were increased by GRF in a concentration-related manner (ED50: 3.5 nM and 6.5 nM, respectively). A significant correlation (r = 0.88, n = 127) was observed between GH and PGE2 release over the range of GRF concentrations tested. Among the five prostanoids analyzed, only PGE2 was selectively increased. Somatostatin lowered GH release, without any effect on PGE2 production. Indomethacin (Id) and Aspirin reduced significantly PGE2 synthesis and GRF-induced GH release. The inhibitory effect of Id was counteracted by addition of PGE2 to the medium. GRF and PGE2, at maximal concentrations, had a partial additive effect on GH release. The increase in PGE2 production and the reduced GH release in the presence of cyclooxygenase inhibitors suggest that PGE2 is involved in GRF-induced GH release.

References

Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P BrazeauR Guillemin

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Citations

Mar 11, 1992·Physiology & Behavior·J M KruegerF Obál
Jun 1, 1989·Prostaglandins, Leukotrienes, and Essential Fatty Acids·A M Cowell, J C Buckingham
Jan 1, 1987·Annals of the New York Academy of Sciences·A B Abou-SamraG Aguilera
Apr 30, 1999·Physiological Reviews·E E MüllerD Cocchi
Dec 12, 2001·Medicine and Science in Sports and Exercise·L Di LuigiD Conte
Nov 1, 1987·The American Journal of Physiology·C SchöflW Knepel
Feb 1, 1989·The American Journal of Physiology·G D SnyderJ R Falck

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