Growth hormone-releasing hormone plasmid treatment by electroporation decreases offspring mortality over three pregnancies

Molecular Therapy : the Journal of the American Society of Gene Therapy
Ryan PersonRuxandra Draghia-Akli

Abstract

LifeTideSW5 is a growth hormone-releasing hormone (GHRH)-expressing plasmid delivered by intramuscular (IM) electroporation (EP), and the first therapeutic plasmid delivered by this physical method to be approved for use in food animals. Gestating sows (n = 997) were treated once with a single 5-mg GHRH-plasmid by EP or served as controls. Data on offspring from three parities subsequent to treatment were collected. No adverse effects related to treatment were noted. First parity post-treatment offspring from treated sows displayed a 2.93 kg (P < 0.0001) increase in carcass weight (CW), 1.0 mm (P < 0.0001) less back-fat (P2), and a 27.0 g CW/day (P < 0.0001) increase in rate of gain (ROG) compared with controls. An increase of 21.6% was recorded in the number of offspring surviving. In the second and third parities post-treatment, offspring from treated females displayed higher number of born alive and total born number, and lower stillborn rates. Third parity offspring from treated sows displayed a 1.6 kg advantage in CW (P < 0.05), 1.0 mm less P2 (P < 0.05), and a 10.0 g CW/day benefit in ROG. Furthermore, offspring from treated females had a 19.04% lower post-wean loss rate. Overall, plasmid GHRH administration decreased mor...Continue Reading

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Citations

Feb 19, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Angela M Bodles-BrakhopRuxandra Draghia-Akli
Sep 3, 2009·Expert Review of Vaccines·Ying CaiHenry Hebel
Aug 7, 2009·Trends in Biotechnology·Pedro H OliveiraGabriel A Monteiro
Jan 5, 2011·Immunological Reviews·Margaret A Liu
Apr 15, 2010·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Sue D XiangMagdalena Plebanski
Mar 2, 2010·Vaccine·Amir S KhanRuxandra Draghia-Akli
Jul 24, 2015·Acta Parasitologica·Halina Wedrychowicz
Jan 30, 2010·Expert Review of Vaccines·Joana A CarvalhoGabriel A Monteiro
Sep 3, 2009·Expert Review of Vaccines·Laurel Redding, David B Weiner
Nov 13, 2020·Frontiers in Bioengineering and Biotechnology·Lalintip HocharoenPanit Kitsubun
Jun 25, 2015·Microbiology Spectrum·Duarte Miguel F Prazeres, Gabriel A Monteiro

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