Growth Hormone-Releasing Hormone Receptor Antagonist Modulates Lung Inflammation and Fibrosis due to Bleomycin

Lung
Chongxu ZhangRobert M Jackson

Abstract

Growth hormone-releasing hormone (GHRH) is a 44-amino acid peptide that regulates growth hormone (GH) secretion. We hypothesized that a GHRH receptor (GHRH-R) antagonist, MIA-602, would inhibit bleomycin-induced lung inflammation and/or fibrosis in C57Bl/6J mice. We tested whether MIA-602 (5 μg or vehicle given subcutaneously [SC] on days 1-21) would decrease lung inflammation (at day 14) and/or fibrosis (at day 28) in mice treated with intraperitoneal (IP) bleomycin (0.8 units on days 1, 3, 7, 10, 14, and 21). Bleomycin resulted in inflammation and fibrosis around airways and vessels evident histologically at days 14 and 28. Inflammation (histopathologic scores assessed blindly) was visibly less evident in mice treated with MIA-602 for 14 days. After 28 days, lung hydroxyproline (HP) content increased significantly in mice treated with vehicle; in contrast, lung HP did not increase significantly compared to naïve controls in mice treated with GHRH-R antagonist. GHRH-R antagonist increased basal and maximal oxygen consumption of cultured lung fibroblasts. Multiple genes related to chemotaxis, IL-1, chemokines, regulation of inflammation, and extracellular signal-regulated kinases (ERK) were upregulated in lungs of mice treated ...Continue Reading

References

Sep 11, 2001·American Journal of Respiratory and Critical Care Medicine·F García-RíoJ Villamor
Oct 19, 2002·Circulation Research·Hideo Kusuoka, Julien I E Hoffman
Sep 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Philip L SimonianAndrew P Fontenot
Dec 18, 2007·Nature Clinical Practice. Endocrinology & Metabolism·Andrew V SchallyJörg B Engel
May 15, 2008·BioTechniques·Ralf-Harto HübnerBurkhard Bewig
Dec 17, 2008·Proceedings of the National Academy of Sciences of the United States of America·Nektarios Barabutis, Andrew V Schally
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
May 3, 2011·Trends in Endocrinology and Metabolism : TEM·Hippokratis KiarisAndrew V Schally
Oct 30, 2012·Bioinformatics·Alexander DobinThomas R Gingeras
Mar 5, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sandra CabreraAnnie Pardo
Apr 17, 2013·BMC Bioinformatics·Edward Y ChenAvi Ma'ayan
Oct 10, 2013·Respiratory Research·Chi F HungLynn M Schnapp
Nov 25, 2014·Methods in Enzymology·Ajit S DivakaruniMartin Jastroch
May 10, 2016·JCI Insight·Samuel L CollinsMaureen R Horton
Jun 15, 2016·Proceedings of the National Academy of Sciences of the United States of America·Muriel ElhaiYannick Allanore
Dec 9, 2016·Proceedings of the National Academy of Sciences of the United States of America·Jinfeng GanHao Zhang
May 2, 2017·American Journal of Respiratory Cell and Molecular Biology·R Gisli JenkinsUNKNOWN ATS Assembly on Respiratory Cell and Molecular Biology
May 20, 2017·Oncotarget·Medhi WangpaichitrNiramol Savaraj
Sep 6, 2017·The Journal of Clinical Investigation·Ying WeiHarold A Chapman
Jan 3, 2018·The Journal of Clinical Investigation·Jeremy HerreraPeter B Bitterman
May 10, 2018·The New England Journal of Medicine·David J Lederer, Fernando J Martinez
Nov 30, 2018·American Journal of Physiology. Lung Cellular and Molecular Physiology·Xinqiang HuangGabor Jarai
Jan 5, 2019·American Journal of Respiratory Cell and Molecular Biology·David W WatersDarryl A Knight
Feb 14, 2019·Annals of the American Thoracic Society·Stefan W RyterAugustine M K Choi

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Citations

Dec 28, 2019·Cell Cycle·Khadeja-Tul KubraNektarios Barabutis
Feb 28, 2020·Cytoskeleton·Nektarios Barabutis
Jan 5, 2020·The Journal of Clinical Endocrinology and Metabolism·Nina Dabrosin, Charlotta Dabrosin
Apr 15, 2020·Clinical and Experimental Pharmacology & Physiology·Nektarios Barabutis
Aug 28, 2020·Frontiers in Medicine·Nektarios Barabutis
Oct 25, 2020·Cells·Chongxu ZhangRobert M Jackson
Oct 4, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Jafrin Jobayer SonjuSeetharama D Jois
Mar 28, 2021·Trends in Endocrinology and Metabolism : TEM·Nektarios Barabutis
Dec 15, 2019·Cellular Signalling·Khadeja-Tul KubraNektarios Barabutis
Apr 3, 2021·Endocrine and Metabolic Science·Nektarios Barabutis

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Methods Mentioned

BETA
RNA-seq

Software Mentioned

Enrichr
edgeR
Trim Galore !
DAVID
BCL2FASTQ
STAR aligner

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