Growth hormone stimulation of the mitogen-activated protein kinase pathway is cell type specific

Endocrinology
D W LoveC M Silva

Abstract

The GH receptor is a member of the cytokine receptor superfamily. Studies in the 3T3-F442A mouse preadipocyte have shown that GH activates the Janus kinase (JAK2), the signal transducers and activators of transcription (STAT1, -3, and -5), and mitogen-activated protein (MAP) kinase. Our previous studies in the human IM-9 lymphocyte have shown that GH activates JAK2 and only STAT5 (not STAT1 or -3). In the studies presented here, we have investigated activation of the MAP kinase (MAPK) pathway in the IM-9 lymphocyte. Western blotting with antiphosphotyrosine-, anti-MAPK-, and anti-phospho-MAPK-specific antibodies as well in vitro kinase assays using a synthetic peptide substrate demonstrate that although GH (200 ng/ml) activates MAPK in 3T3-F442A cells (at 5 and 10 min of treatment), it does not activate MAPK in IM-9 lymphocytes at time points ranging from 5-60 min. Nevertheless, the phorbol ester phorbol 12-myristate 13-acetate (50 ng/ml) does activate MAPK in the IM-9 cell, and immunoprecipitation with specific antibodies indicates that this activation occurs through c-Raf-1. Although the 52- and 66-kDa forms of the adapter protein Shc are tyrosine phosphorylated in response to GH treatment in 3T3-F442A cells, we demonstrate t...Continue Reading

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Citations

Nov 19, 2004·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Joachim WoelflePeter Rotwein
Oct 22, 2003·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Alexander Y. ZubkovJohn H. Zhang
Oct 6, 2009·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Archana VijayakumarDerek LeRoith
Oct 13, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Panos G ZirosAthanasios G Papavassiliou
May 2, 2003·British Journal of Pharmacology·Laurence LempereurGiuseppina Cantarella
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Mar 7, 2006·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Xiaoyu WuJieshou Li
Jun 2, 2009·Expert Opinion on Investigational Drugs·Gui Tong TranEleftherios Tsiridis
Nov 26, 2002·Endocrinology·Sung-Oh KimStuart J Frank
Jun 20, 2002·Endocrinology·Corinne M SilvaPeter E Clayton
Dec 10, 1999·The Journal of Biological Chemistry·S O KimS J Frank
Aug 6, 2002·The Journal of Biological Chemistry·Shaonin JiJoseph L Messina
Aug 25, 2020·Molecular and Cellular Endocrinology·Stuart J Frank
Sep 24, 2020·Molecular and Cellular Endocrinology·Rita SharmaVishva M Sharma
Aug 10, 2001·Cellular Signalling·T ZhuP E Lobie

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