PMID: 9178824May 16, 1997Paper

Growth inhibition and apoptotic cell death in uterine cervical carcinoma cells induced by 5-fluorouracil

International Journal of Cancer. Journal International Du Cancer
M UedaM Ueki

Abstract

We have investigated the effects of 5-fluorouracil (5-FU) on cell growth, DNA synthesis, morphological changes, DNA fragmentation and Fas antigen expression of cultured human uterine cervical carcinoma cells (OMC-1 squamous-cell carcinoma and OMC-4 adenocarcinoma). Apoptotic cell death in cervical carcinoma tissues from the patients after an intravenous administration of 5-FU was also examined. Treatment of OMC-1 and OMC-4 cells with 0.1-10 microg/ml of 5-FU (1 microg/ml = 7.7 microM) resulted in concentration-dependent inhibition of the number of cumulative viable cells and 6-3H-deoxyuridine uptake into the DNA. These effects of 5-FU were well correlated with apoptotic indices of the cells determined in situ by terminal deoxynucleotidyl transferase-mediated digoxigenin-dUTP nick end-labeling (TUNEL). A TUNEL signal was detectable in nuclei of normal-looking cells and in a lobulated nucleus with dense chromatin. DNA fragmentation was observed in the cells exposed to 10 microg/ml of 5-FU according to the nucleosomal ladder detected by electrophoresis. Flow cytometric analysis showed that Fas antigen expression of the cells increased upon incubation with 10 microg/ml of 5-FU. Moreover, TUNEL of tissue sections of resected uterus ...Continue Reading

References

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Citations

Jul 13, 2000·Biochemical and Biophysical Research Communications·K AotaM Sato
Oct 25, 2002·Cancer Biotherapy & Radiopharmaceuticals·Nobuhiro ItoToshiaki Nonami
May 1, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Megan M GarrityUNKNOWN North Central Cancer Treatment Group
Jul 8, 2003·International Journal of Cancer. Journal International Du Cancer·Masayasu IwaseMasao Nagumo
Aug 29, 2002·Cancer Investigation·Athanasios RigasBasil Rigas
Oct 27, 2017·Journal of Theoretical Biology·Dan E GanzNeil S Forbes
Feb 19, 2000·Biochemical and Biophysical Research Communications·D E ModrakR Blumenthal

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