Growth Inhibition of Cronobacter sakazakii in Experimentally Contaminated Powdered Infant Formula by Kefir Supernatant

Journal of Food Protection
Dong-Hyeon KimKun-Ho Seo

Abstract

Kefir is a type of fermented milk containing lactic and acetic acid bacteria and yeast. In this study, we evaluated the antimicrobial activity of kefir supernatant against Cronobacter sakazakii in powdered infant formula (PIF). In a spot-on-lawn test, the growth of 20 C. sakazakii strains, including 10 clinical and 10 food isolates, was completely inhibited in the presence of kefir supernatant. Significant differences in the diameters of inhibition zones were observed upon treatment with kefir compared with the results for Lactobacillus kefiri and Candida kefyr culture supernatants or solutions of lactic and acetic acid and ethyl alcohol in the agar well diffusion test (P < 0.05). The addition of 100 μl of kefir supernatant to 1 ml of nutrient broth completely inhibited the growth of C. sakazakii, as evaluated by spectrophotometry. The antimicrobial activity of kefir supernatant in experimentally contaminated PIF was also tested; we found no viable C. sakazakii cells remaining in PIF rehydrated with 30% kefir supernatant solution for 1 h, demonstrating that the antimicrobial activity of kefir supernatant against C. sakazakii could be applied in real food samples.

References

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Sep 1, 2009·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Gaetano ChiricoClaudia Chirico
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Mar 11, 2011·Critical Reviews in Food Science and Nutrition·Zeynep B Guzel-SeydimAtif C Seydim

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Citations

Jan 25, 2017·Korean Journal for Food Science of Animal Resources·Dong-Hyeon KimKun-Ho Seo
Jan 18, 2018·Critical Reviews in Food Science and Nutrition·Dong-Hyeon KimKun-Ho Seo
Jun 5, 2019·Nutrients·Conor SlatteryPaul W O'Toole
May 6, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Abdul-Raouf Al-MohammadiGamal Enan

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