Growth-inhibitory and differentiation-inducing activity of dimethylformamide in cultured human malignant glioma cells

Neurosurgery
X N LiJ Q Wu

Abstract

To determine the growth-inhibitory and differentiation-inducing activity of dimethylformamide (DMF) on a human glioma cell line (SHG-44). DMF is a type of polar solvent and a potent differentiation-inducing agent in many kinds of human solid tumors, yet its effect on human glioma remains unclear. The effects of DMF on cell proliferation using 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, cell cycle distribution (with flow cytometry), colony-forming efficiency in double-layer soft agar, tumorigenicity in athymic nude mice, morphological changes, and glial fibrillary acidic protein expression were studied. At dose ranges of 0.25, 0.5, 0.75, and 1.0%, DMF caused a dose-dependent proliferation inhibitory effect in monolayers and a marked dose-dependent suppression of colony-forming efficiency in double-layer soft agar with a complete loss of colony-forming ability in cells exposed to 0.75 and 1.0% DMF. Accumulation of cells in G0/G1 phases was observed in DMF-treated (0.5 and 1.0%) cells, also in a dose-dependent manner. SHG-44 cells exposed to DMF (0.5 and 1.0%) for 15 days changed morphologically from small spindle-shaped to large polygonal and flattened stellate cells with multiple slender processes. Thes...Continue Reading

References

Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A S RabsonJ Wilczek
Mar 1, 1979·Journal of the National Cancer Institute·C M JacqueN Baumann
Oct 1, 1990·Journal of Cellular Physiology·N F GuilbaudA Valette
Feb 15, 1989·International Journal of Cancer. Journal International Du Cancer·G A van DongenG B Snow
Sep 15, 1988·International Journal of Cancer. Journal International Du Cancer·J T RutkaM L Rosenblum
Jan 1, 1984·Journal of Neurochemistry·J E Goldman, F C Chiu
Sep 1, 1994·Journal of Neurosurgery·S TanakaS Yasumoto
Jan 2, 1993·International Journal of Cancer. Journal International Du Cancer·T DohiM Oshima

❮ Previous
Next ❯

Citations

Dec 17, 2009·Evidence-based Complementary and Alternative Medicine : ECAM·Hardeep KatariaGurcharan Kaur
Oct 26, 2013·Journal of Molecular Neuroscience : MN·Lijun SunDefu Chen
Jul 25, 2012·Journal of Cancer Research and Clinical Oncology·Jun DongQiang Huang
Apr 15, 2008·Life Sciences·Young-Gi Gil, Mi-Kyung Kang
Nov 7, 2017·Pharmaceutical Development and Technology·Wipada SamprasitPraneet Opanasopit
Dec 9, 2014·Cancer Biology & Therapy·Laurence BoothPaul Dent
Jul 12, 2018·Cancer Biomarkers : Section a of Disease Markers·Xiaoyan JiJun Dong
Jun 15, 2006·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Xin-Xia TianJie Zheng
Oct 15, 2008·Journal of Cancer Research and Clinical Oncology·Hui-zhi FanPeng-yuan Yang
Feb 12, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Xiao-Nan LiChing C Lau
Mar 22, 2021·The Journal of Biological Chemistry·Matías Arturo PibuelSilvina Laura Lompardía

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.