Growth kinetics and cellulase biosynthesis in the continuous culture of Trichoderma viride

Biotechnology and Bioengineering
D E Brown, M A Zainudeen

Abstract

Continuous culture studies have been carried out growing Trichoderma viride QM 9123 in a 10 liter stirred fermentor on a medium containing commercial glucose as the carbon source. Experiments were carried out at 30 degrees C and at three controlled pH values of 2.5, 3.0, and 4.0 over a range of dilution rates from 0.01 to 0.11 hr-1. Steady-state values of cell, glucose, and cellulase concentration oxygen tension, and outlet gas oxygen partial pressure were recorded. Values of maximum specific growth rate, endogenous metabolism coefficient, Michaelis-Menten coefficient, yield and maintenance coefficient for glucose were derived and correlated the effect of the hydrogen ion concentration. Specific oxygen uptake rates were correlated with specific growth rates and absorption coefficients were shown to be a function of dilution rate independent of pH. Some data on cellulase biosynthesis were examined and correlated in terms of a maturation time model.

References

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Citations

Apr 5, 1992·Biotechnology and Bioengineering·D W Schafner, R T Toledo
Sep 5, 1995·Biotechnology and Bioengineering·R LejeuneG V Baron
May 29, 2013·World Journal of Microbiology & Biotechnology·Divanery Rodriguez-Gomez, Timothy John Hobley
Dec 11, 2003·Biotechnology Advances·Maria Papagianni
Apr 25, 1997·Journal of Biotechnology·S VelkovskaD F Ollis
Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Lee R LyndIsak S Pretorius

Related Concepts

Endoglucanase Y
Hyphomycetes
Hydrogen-Ion Concentration
Oxygen Consumption
Trichoderma

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