PMID: 6537788Jan 1, 1984Paper

Growth kinetics, cell shape, and the cytoskeleton of primary astrocyte cultures

Journal of Neurochemistry
J E Goldman, F C Chiu

Abstract

We examined correlations among growth kinetics, cell shape, and cytoskeletal protein content in rat astrocytes grown in primary culture. Cell suspensions from brains of newborn rats were seeded at densities from 0.2 to 3 X 10(5)/cm2. At initial densities above 1 X 10(5) the population increased to reach confluency by 10-12 days, after which cell number remained stable for many weeks. At low initial densities, 0.2-0.4 X 10(5)/cm2, cells did not increase in number. Final density increased with increasing plating densities. High-density cells had small perikarya and several long cytoplasmic processes; low-density cells appeared flat and polygonal. All cultures were almost entirely astrocytic, as judged by immunofluorescent staining with antiserum against glial fibrillary acidic protein (GFAP). Cytoskeletal proteins were analyzed by gel electrophoresis after extraction from cells with nonionic detergent. Relative amounts of the proteins differed, in that low-density cells contained large amounts of cytoskeletal actin relative to the intermediate filament (IF) proteins vimentin and GFAP, whereas high-density cells contained relatively less actin and more IF proteins. Such differences in cytoskeletal proteins between the high- and lo...Continue Reading

References

Dec 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L A Sternberger
Aug 1, 1978·The Journal of Cell Biology·J E GoldmanW T Norton
May 7, 1971·Brain Research·L F EngB Gerstl
Feb 1, 1971·European Journal of Biochemistry·U Lindberg, S Eriksson
Jun 1, 1980·The Journal of Cell Biology·K D McCarthy, J de Vellis
Apr 1, 1981·Neurochemical Research·F P White, L Hertz
Jan 1, 1984·Journal of Neurochemistry·F C Chiu, J E Goldman
Apr 21, 1980·Brain Research·E HanssonL Rönnbäck
Nov 1, 1981·Journal of Neurochemistry·L Autilio-GambettiP Gambetti

❮ Previous
Next ❯

Citations

Jan 1, 1993·Acta Neuropathologica·M MiyazonoJ Tateishi
Dec 1, 1984·Neurochemical Research·M V Frangakis, H K Kimelberg
Dec 1, 1986·Journal of the Neurological Sciences·H KoppelP L Lantos
Aug 1, 1989·Brain Research. Developmental Brain Research·C PetersonC W Cotman
Apr 16, 1993·Brain Research. Developmental Brain Research·W T Norton, M Farooq
May 1, 1990·Brain Research. Molecular Brain Research·M BrennerE Freese
Aug 1, 1987·Neuroscience·O B IlyinskyZ D Bespalova
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M R Freeman, N Sueoka
May 8, 1999·Molecular Biology of the Cell·D KalmanJ M Bishop
Jun 1, 1995·Neuropathology and Applied Neurobiology·Y HiguchiJ Tateishi
Sep 1, 1997·Journal of Neurosurgery·J T RutkaK Matsuzawa
Sep 1, 1996·Molecular and Chemical Neuropathology·P G LalL F Eng
Jan 17, 2009·Experimental Cell Research·Woosung Cho, Albee Messing
May 26, 2005·Brain Research. Brain Research Reviews·Lyle W Ostrow, Frederick Sachs
Jan 1, 1985·Annals of the New York Academy of Sciences·L F EngR P Skoff
Jan 1, 1984·Journal of Neurochemistry·F C Chiu, J E Goldman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.