PMID: 16535796Mar 16, 2006Paper

Growth of compound layer tissue engineered oral mucosa and its clinical application in hetero-transplantation

Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery
Hongwei LiChangmei Wang

Abstract

To investigate the growth of the tissue engineered mucosa after its hetero-transplantation. The epithelial cells and fibroblasts were isolated from a postoperative tissue of the 3-month patient with labial cleft. The epithelial cells and fibroblasts were separately seeded on the polylactic/glycolic acid copolymer membrane, and then they were exposed to the air-liquid interface. Seven volunteer patients, whose traumatic beds were repaired with the tissue engineered oral mucosa. The biopsy tissue from one of the seven patients was observed under light microscope 18 and 30 days after transplantation, respectively. The tissue engineered oral mucosa having 5-6 layers anti-cytokeratin staining positively cells in the epithelial layer and 3-7 layers anti-Cytokeratin staining negatively cells in the subepithelial layer grew well after the hetero-transplantation. No difference could be found between the transplanting and normal areas. At 18 days, the epithelial layer and lamina propria grew well and the fibroblasts were found; at 30 days, collagen was obviously observed. The structures in both the transplanting and the normal areas were similar. The tissue engineered oral mucosa can grow well after the hetero-transplantation.

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