Growth of Ehrlichia canis, the causative agent of canine monocytic ehrlichiosis, in vector and non-vector ixodid tick cell lines

Ticks and Tick-borne Diseases
Joana FerrolhoL Bell-Sakyi

Abstract

Canine monocytic ehrlichiosis is caused by Ehrlichia canis, a small gram-negative coccoid bacterium that infects circulating monocytes. The disease is transmitted by the brown dog tick Rhipicephalus sanguineus s.l. and is acknowledged as an important infectious disease of dogs and other members of the family Canidae worldwide. E. canis is routinely cultured in vitro in the canine monocyte-macrophage cell line DH82 and in non-vector Ixodes scapularis tick cell lines, but not in cells derived from its natural vector. Here we report infection and limited propagation of E. canis in the tick cell line RSE8 derived from the vector R. sanguineus s.l., and successful propagation through six passages in a cell line derived from the experimental vector Dermacentor variabilis. In addition, using bacteria semi-purified from I. scapularis cells we attempted to infect a panel of cell lines derived from non-vector species of the tick genera Amblyomma, Dermacentor, Hyalomma, Ixodes and Rhipicephalus with E. canis and, for comparison, the closely-related Ehrlichia ruminantium, causative agent of heartwater in ruminants. Amblyomma and non-vector Dermacentor spp. cell lines appeared refractory to infection with E. canis but supported growth of E....Continue Reading

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Sep 4, 2013·Veterinary Microbiology·Alejandro Cabezas-CruzLygia Maria Friche Passos
Jul 27, 2015·Veterinary Microbiology·Lesley Bell-SakyiVarda Shkap

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Citations

Mar 18, 2015·PloS One·Geoffrey E LynnUlrike G Munderloh
Sep 18, 2018·Vector Borne and Zoonotic Diseases·Darci Moraes Barros-BattestiMatias Pablo Juan Szabó
Jun 25, 2019·Transboundary and Emerging Diseases·Filipa DiasAna Domingos
Aug 12, 2021·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Mayara de Cassia LuzziDarci Moraes Barros-Battesti
Aug 28, 2021·Animals : an Open Access Journal From MDPI·Boondarika NamboopphaNattawooti Sthitmatee

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Methods Mentioned

BETA
PCR
electrophoresis
transmission electron microscopy

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