Growth of Escherichia coli MG1655 on LB medium: monitoring utilization of sugars, alcohols, and organic acids with transcriptional microarrays

Applied Microbiology and Biotechnology
Mark V BaevJohn W Campbell

Abstract

Microorganisms respond to environmental changes by reprogramming their metabolism primarily through altered patterns of gene expression. DNA microarrays provide a tool for exploiting microorganisms as living sensors of their environment. The potential of DNA microarrays to reflect availability of nutrient components during fermentations on complex media was examined by monitoring global gene expression throughout batch cultivation of Escherichia coli MG1655 on Luria-Bertani (LB) medium. Gene expression profiles group into pathways that clearly demonstrate the metabolic changes occurring in the course of fermentation. Functional analysis of the gene expression related to metabolism of sugars, alcohols, and organic acids revealed that E. coli growing on LB medium switches from a sequential mode of substrate utilization to the simultaneous one in the course of the growth. Maltose and maltodextrins are the first of these substrates to support growth. Utilization of these nutrients associated with the highest growth rate of the culture was followed by simultaneous induction of enzymes involved in assimilation of a large group of other carbon sources including D-mannose, melibiose, D-galactose, L-fucose, L-rhamnose, D-mannitol, amino...Continue Reading

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Citations

Jul 5, 2013·Archives of Microbiology·Muriel B Herrington, Chris Sitaras
Apr 29, 2006·Applied Microbiology and Biotechnology·Mark V BaevJohn W Campbell
Dec 26, 2007·Molecular Genetics and Genomics : MGG·Tao DongHerb E Schellhorn
Jul 2, 2011·Molecular & Cellular Proteomics : MCP·Francesca GubelliniJohn F Hunt
Dec 4, 2012·Applied and Environmental Microbiology·Pilar Eliana Puentes-TéllezJan Dirk van Elsas
Jan 18, 2014·Analytical Biochemistry·V Durán-ToroJ M Pérez-Donoso
Jul 11, 2014·Antonie van Leeuwenhoek·Pilar Eliana Puentes-Téllez, Jan Dirk van Elsas
Oct 23, 2012·Journal of Bioscience and Bioengineering·Zoe SanchezKazuhide Kimbara
Feb 2, 2011·Environmental Microbiology·Paola MilanesioVíctor de Lorenzo
Jun 1, 2011·Environmental Microbiology Reports·Pilar FonsecaFernando Rojo
Jul 1, 2014·Environmental Microbiology·Pilar Eliana Puentes-Téllez, Jan Dirk van Elsas
Jan 8, 2017·BMC Microbiology·Lucia Gonzales-SilesÅsa Sjöling
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Sep 11, 2007·Rapid Communications in Mass Spectrometry : RCM·Delphine OurselCatherine M Lange
Sep 17, 2013·Environmental Microbiology·Pilar Eliana Puentes-TéllezJan Dirk van Elsas
Jan 8, 2020·Journal of Biophotonics·Diganta HatiboruahPabitra Nath
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Nov 17, 2020·Frontiers in Cellular and Infection Microbiology·Congmin XuLiping Duan
Apr 24, 2021·Nature Communications·Alan R PachecoDaniel Segrè
Jul 5, 2021·Colloids and Surfaces. B, Biointerfaces·André Pawlak, Sabrina Belbekhouche

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