Growth of fetal thymocytes in organ culture: effect of recombinant lymphokines on thymocyte maturation.

Growth Factors
P S CrosierJ D Watson

Abstract

Interleukin 2 (IL2) has a dose-dependent inhibitory effect on the growth and phenotypic maturation of thymocyte populations grown in fetal thymus organ culture. Addition of IL2 (100 U/ml) to 14-day fetal thymus organ cultures induces the appearance of a population of lymphokine-activated killer (LAK) cells which lyse allogeneic, syngeneic, and syngeneic tumor cell targets. The addition of the monoclonal antibody, PC-61, blocks the IL2-dependent growth and activation of LAK cells but does not influence the maturation of CD4+ CD8+ fetal thymocytes. These data imply that IL2 is not a major regulator of normal fetal thymocyte maturation. The effects of a range of recombinant lymphokines (IL1 alpha, IL1 beta, IL3, IL4, GM-CSF, G-CSF, M-CSF) on the proliferation and phenotypic maturation of fetal 14-day thymocytes in organ culture has been analysed. Two significant changes were seen. First, IL1 alpha and IL1 beta inhibited growth and the expression of the CD4 and CD8 antigens in organ culture, and second, GM-CSF increased the expression of Mac-1+ cells. IL4, which has known T cell growth-promoting activity, IL3, G-CSF, and M-CSF did not alter either normal growth or surface antigen expression in fetal thymocytes. While some of these ...Continue Reading

References

Jan 1, 1979·Immunological Reviews·J A Ledbetter, L A Herzenberg
Dec 1, 1987·The Journal of Experimental Medicine·E A Kurt-JonesA K Abbas
Mar 7, 1985·Nature·D H RauletS Tonegawa
Jan 1, 1986·European Journal of Immunology·R Palacios, H Von Boehmer
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T R MosmannW E Paul
Jan 1, 1987·The Journal of Experimental Medicine·K TeshigawaraK A Smith
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M TsudoT A Waldmann
Jun 1, 1987·The Journal of Experimental Medicine·M SkinnerJ D Watson
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M TsudoT A Waldmann
Sep 24, 1987·Nature·I N CrispeR P Shimonkevitz
Apr 24, 1987·Cell·J W KapplerP Marrack
Jul 1, 1987·International Journal of Cell Cloning·M TamirD Zipori
Feb 14, 1985·Nature·H R SnodgrassH von Boehmer
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T R MalekE M Shevach
Nov 1, 1981·The Journal of Experimental Medicine·R J RobbK A Smith
Feb 1, 1982·The Journal of Experimental Medicine·R CeredigJ J Owen

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Citations

May 1, 1992·International Journal of Cell Cloning·J D Watson, H J McKenna
May 16, 2009·Developmental and Comparative Immunology·Daphne A van HaarlemLonneke Vervelde
May 16, 2002·Blood·Terry J Fry, Crystal L Mackall
Jan 31, 1998·The Journal of Experimental Medicine·Q LinM D Cooper
Jan 1, 1990·International Journal of Cell Cloning·M B WidmerA E Namen
Jun 28, 2016·Experimental and Therapeutic Medicine·Annie BartlettWen G Jiang
Oct 1, 1996·The British Journal of Dermatology·M BagotJ Wechsler

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