Growth of lactic acid bacteria in milk phospholipids enhances their adhesion to Caco-2 cells

Journal of Dairy Science
Diana Rocha-MendozaIsrael García-Cano

Abstract

The mechanisms of bacterial adhesion to human cells involve several complex reactions and activation of genes and proteins. It has been reported that the food components in dairy matrices, such as sugar or salt, can decrease bacterial adhesion to Caco-2 cells. However, it has not been evaluated whether the bacteria grown in media supplemented with milk phospholipids (MPL) can increase or decrease the adhesion of these cells. The objective of this work was to evaluate the effects of MPL on the kinetic growth of lactic acid bacteria (LAB) and their functional characteristics as probiotics, expression of surface protein genes, and adherence to Caco-2 cells. Seven LAB strains isolated from various dairy products were characterized. Five of the tested LAB strains were able to grow in a chemically defined medium supplemented with MPL. Lactobacillus reuteri OSU-PECh-48 showed the highest growth rate and the greatest optical density. All of the strains tested showed tolerance to acidic conditions at pH 3.0 and to bile salts at 0.5 and 1% concentrations. Auto-aggregation and cell surface hydrophobicity ability were evaluated, with nonsignificant differences between the strains grown in MPL and without MPL. Gene expression of 6 surface p...Continue Reading

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Jul 15, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gonzalo Miyagusuku-CruzadoM Monica Giusti
Aug 10, 2021·Journal of Agricultural and Food Chemistry·Yunping YaoShuo Wang

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