Growth of molluscum contagiosum virus in a human foreskin xenograft model

Virology
K H FifeD R Brown

Abstract

Molluscum contagiosum virus (MCV) is a common pathogen causing a troublesome skin condition in many immunocompetent individuals and a widespread, disfiguring affliction in many patients with AIDS. We have successfully infected human foreskin fragments with a patient-derived isolate of MCV. This was demonstrated by exposing the foreskin pieces to a patient lesion extract and implanting the tissue under the renal capsule of athymic mice. Light and electron microscopic examination of infected implants showed the presence of cytoplasmic inclusions containing typical poxvirus particles within 2-3 weeks of implantation. Replication of MCV was established by demonstrating that viable virus was required to produce the cytopathologic effects, and viral DNA replication was demonstrated by incorporation of bromodeoxyuridine into cytoplasmic inclusions. Four additional patient extracts (representing both described MCV types) were also used to successfully infect foreskin implants. A limited number of attempts to pass virus from one infected implant to another were not successful. This system is the most rapid and reproducible for growing MCV that has been reported to date.

Citations

Feb 22, 2014·PloS One·Subuhi SherwaniJoachim Jakob Bugert
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·I DamonB Moss
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y Xiang, B Moss
Sep 2, 1997·Proceedings of the National Academy of Sciences of the United States of America·M D KrathwohlK H Fife
Jun 13, 1998·Research in Veterinary Science·C H ThompsonI B Van Rensburg
Oct 13, 2006·Current Opinion in Infectious Diseases·C H Thompson
Feb 1, 2006·International Journal of Dermatology·Justin BrownNanette B Silverberg
Aug 27, 2013·The Lancet Infectious Diseases·Xiaoying ChenJoachim J Bugert
Jan 10, 2001·Journal of the American Academy of Dermatology·D G Diven

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