PMID: 6969888Nov 1, 1980Paper

Growth of normal human glial cells in a defined medium containing platelet-derived growth factor

Proceedings of the National Academy of Sciences of the United States of America
C H HeldinB Westermark

Abstract

DNA synthesis and cell division were measured in serum-free cultures of human normal diploid glial cells maintained in MCDB 105 medium. In growth factor-free cultures the cells remained viable but the cell number was essentially constant. Supplementation with 10 ng of epidermal growth factor or platelet-derived growth factor per ml significantly stimulated DNA synthesis and cell multiplication. Growth occurred both when cells were allowed to settle in serum-containing medium and when cells were plated in serum-free medium. In the latter type of cultures, the cell yield was improved bu incubating the cells in collagen-coated dishes. The use of a miniclone technique allowed the analysis of cell multiplication induced by platelet-derived growth factor at the clonal level and demonstrated that the growth factor induced several cell cycle rounds in a large fraction of clones. The results show that normal cells grown in a recently developed synthetic medium (MCDB 105) supplemented with pure growth factors may multiply without the addition of plasma-derived factors ("progression factors"). It is suggested that the need fo progression factors may simply depend on the composition of the synthetic nutrient medium.

References

Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·W J PledgerC D Scher
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A VogelR Ross
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·W J PledgerC D Scher
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H N AntoniadesC D Stiles
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·C H HeldinA Wasteson
Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·C D StilesW J Pledger
Feb 1, 1978·Experimental Cell Research·B Westermark
Jun 1, 1978·Cell·R Ross, A Vogel
Aug 10, 1979·Biochimica Et Biophysica Acta·C D ScherC D Stiles
Feb 28, 1978·Biochemical and Biophysical Research Communications·W L McKeehanR G Ham
Nov 1, 1978·Experimental Cell Research·K RubinB Obrink
Mar 22, 1976·Biochemical and Biophysical Research Communications·B Westermark
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R RossL Harker
Aug 1, 1974·Experimental Cell Research·N Kohler, A Lipton
Dec 1, 1969·Experimental Cell Research·J PonténR Hugosson
Oct 1, 1980·Experimental Cell Research·J Pontén, L Stolt

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Citations

Nov 23, 1981·Life Sciences·D S Straus
Mar 1, 1985·Molecular and Cellular Endocrinology·C H HeldinB Westermark
May 1, 1986·Brain Research Bulletin·C Estin, A Vernadakis
Jan 1, 1984·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·A Michler-StukeJ E Bottenstein
Jan 1, 1990·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M Taub
Feb 18, 1998·Brain Research. Developmental Brain Research·P E SpoerriA Vernadakis
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R S Morrison, J de Vellis
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·W J PledgerC D Scher
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S D BalkN M Raymond
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M E KaighnJ F Lechner
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A J OwenH N Antoniades
Jan 17, 1995·International Journal of Cancer. Journal International Du Cancer·A GuhaC D Stiles
Nov 24, 2005·Journal of Tissue Viability·Nicola C PetrieElof Eriksson
Apr 1, 1992·Surgical Neurology·K WatanabeJ Handa
Oct 22, 2004·Seminars in Oncology·Ravi D Rao, C David James
Oct 1, 1992·Pathology·L M Khachigian, C N Chesterman
Jan 1, 1982·Journal of Neuroscience Research·R S MorrisonJ de Vellis
Jan 1, 1982·Journal of Neuroscience Research·A Fontana
Oct 1, 1983·Journal of Muscle Research and Cell Motility·K MellstroömU Lindberg

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