Growth of pure cultures of Verocytotoxin-producing Escherichia coli in a range of enrichment media

Journal of Applied Microbiology
C L Baylis

Abstract

This study compared the growth of different strains of Verocytotoxin-producing Escherichia coli (VTEC) in a range of selective enrichment media. Turbidometric and impedance methods were used to determine the growth of VTEC in pure culture in different enrichment media. Ten strains failed to grow in buffered peptone water + vancomycin, cefsulodin, cefixime at 42 degrees C and some failed to grow, or grew poorly in E. coli (EC) medium supplemented with 20 mg l(-1) novobiocin and modified EC supplemented with 20 mg l(-1) novobiocin at 37 degrees C and 42 degrees C. Individual VTEC strains were sensitive to the selective agents in some media. Statistical analysis of the conductance detection times of 10 strains showed no overall effect of temperature alone (P = 0.66) but there were significant (P < 0.001) effects as a result of the combination of medium and temperature and these two factors were influenced by strain. Growth of VTEC during enrichment is dependent on different factors alone or in combination. These include medium type, presence of certain selective agents or antibiotics, incubation temperature and the initial population of VTEC. Sensitivity to these conditions can be strain related. This study highlighted differences...Continue Reading

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Citations

Jun 13, 2013·Foodborne Pathogens and Disease·Fei WangBeilei Ge
Nov 20, 2012·Applied and Environmental Microbiology·Dominic FrigonRonald Gehr
Sep 4, 2012·International Journal of Food Microbiology·Choreh FarrokhOlivier Cerf
Jan 7, 2016·International Journal of Food Microbiology·Bavo VerhaegenKoen De Reu
Apr 29, 2015·Current Microbiology·Zachary R StrombergRodney A Moxley
Sep 20, 2015·Applied and Environmental Microbiology·Susan R LeonardChristopher A Elkins
May 9, 2012·Journal of Food Protection·Alexander GillBurton Blais
Nov 13, 2013·Journal of Environmental Quality·Lisa M Durso
Nov 1, 2017·Comprehensive Reviews in Food Science and Food Safety·Vinicius Silva CastroEduardo Eustáquio Souza Figuiredo
Jun 25, 2015·Microbiology Spectrum·Lothar Beutin, Patrick Fach

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