PMID: 7542686Aug 1, 1995Paper

Growth of Staphylococcus aureus with nafcillin in vitro induces alpha-toxin production and increases the lethal activity of sterile broth filtrates in a murine model

The Journal of Infectious Diseases
D S KernodleS Harshman

Abstract

The morbidity and mortality of Staphylococcus aureus infections remain high despite antibiotic therapy. To investigate further the observation that penicillins increase the hemolytic activity of staphylococcal cultures, 37 strains were grown in broth with and without subinhibitory nafcillin. Nafcillin stimulated hemolytic activity in nafcillin-susceptible and -resistant isolates. Sterile broth filtrates of nafcillin-associated cultures injected intraperitoneally in mice were more rapidly lethal than filtrates of the same strain grown without nafcillin. Lethality was neutralized by anti-alpha-toxin antisera. DNA-RNA hybridization revealed a nafcillin-associated increase in alpha-toxin mRNA during the postexponential growth phase after the activation of agr. Isolates grown in slightly inhibitory nafcillin concentrations had more alpha-toxin mRNA than did nafcillin-free cultures, whereas agr RNAIII levels were comparable. This suggests that nafcillin-induced alpha-toxin production is not entirely attributable to agr. A supplemental regulatory mechanism may be involved.

Citations

Mar 27, 2013·Paediatric Drugs·Amanda K Sullins, Susan M Abdel-Rahman
Sep 1, 1996·American Journal of Surgery·R T Villavicencio, M J Wall
Jun 14, 2003·The Journal of Alternative and Complementary Medicine : Research on Paradigm, Practice, and Policy·Theodor DanningerJutta Kraeling
Apr 27, 2013·Anaerobe·Luciano de Castro VelosoMaria Auxiliadora Roque de Carvalho
Nov 4, 2016·Medical Microbiology and Immunology·Manikandan KathirvelMrinalini Nair
Jul 21, 2017·Clinical Microbiology Reviews·Elisabeth HodilleOana Dumitrescu
Apr 26, 2019·Current Opinion in Infectious Diseases·Jessica J Evans, Devin D Bolz
Dec 31, 2003·The Journal of Biological Chemistry·Carmelo BisognanoPierre Vaudaux
Sep 26, 2006·Journal of Cellular Physiology·Barbara A E de KoningIngrid B Renes
Oct 6, 2007·The International Journal of Artificial Organs·L BaldassarriC R Arciola
Jan 13, 2017·The Journal of Infectious Diseases·Arnold S Bayer, Yan Q Xiong
Sep 29, 2004·The Lancet Infectious Diseases·Stephanie J Dancer

❮ Previous
Next ❯

Related Concepts

Related Feeds

CRISPR & Staphylococcus

CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.

Allergy & Infectious Diseases

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Allergy & Infectious Diseases (ASM)

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Related Papers

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
A W BerringtonF K Gould
© 2021 Meta ULC. All rights reserved