Growth patterns of primary cultures dissociated from 3-day-old chick embryos: morphological and biochemical comparisons

Journal of Neuroscience Research
A VernadakisD Mangoura

Abstract

Cultures were prepared by dissociating 3-day-old whole chick embryos and plating the dispersed cells on poly-L-lysine-coated dishes in Dulbecco's Modified Eagle's Medium with 10% fetal calf serum. By 48 hr in culture, aggregates and neuritic sprouting were observed. Long neuritic bundles connecting cell aggregates were evident by 4 days in culture. Consistent patterns throughout the lifespan of the cultures were contacts between neurites, and flat isolated cells, presumptively glial, emerged. Throughout the lifespan of the cultures, the cholinergic cell population was characterized histochemically by the method of Karnovsky and Roots and biochemically by assaying choline acetyltransferase. By 4 days in culture, all aggregates showed light cholinesterase-positive staining; however, with days in culture, several aggregates had no staining, and some positive-stained aggregates were interconnected with other aggregates showing only spotted positive staining. Choline acetyltransferase activity showed a developmental profile in agreement with the histological findings. The early presence of choline acetyltransferase activity is taken as indication of the early commitment of cholinergic neurons.

References

Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·F Collins
Sep 3, 1979·Cell and Tissue Research·J R WolffB M Chronwall
Dec 1, 1979·The Journal of Comparative Neurology·M ColonnierH McLennan
Sep 1, 1975·Experimental Neurology·R L Sidman, N K Wessells
Oct 7, 1977·Science·A M Heacock, B W Agranoff
Mar 1, 1975·The Journal of Comparative Neurology·F F Ebner, M Colonnier
Sep 20, 1974·Science·D McMahon
Jan 1, 1971·Neuroscience Research·G Filogamo, P C Marchisio
Jun 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·D MonardR Gysin
Sep 1, 1970·Experimental Cell Research·T Gustafson, M Toneby
Jan 1, 1968·Zeitschrift für Zellforschung und mikroskopische Anatomie·F GraingerR L Tresman
Oct 1, 1984·Cell Differentiation·J E Valinsky, C Loomis
Jan 1, 1982·Journal of Neuroscience Research·E D Pollack, W L Muhlach
Jan 1, 1981·Advances in Experimental Medicine and Biology·J M LauderH Krebs
Mar 1, 1964·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M J KARNOVSKY, L ROOTS

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Citations

Jun 1, 1996·Journal of Neuroscience Research·P E SpoerriA Vernadakis
Jun 15, 1996·Journal of Neuroscience Research·S Kentroti, A Vernadakis
Jul 1, 1990·Journal of Neuroscience Research·A Vernadakis, S Kentroti
Oct 1, 1989·Brain Research. Developmental Brain Research·S Kentroti, A Vernadakis
Nov 1, 1990·Brain Research. Developmental Brain Research·S Kentroti, A Vernadakis
Nov 1, 1990·Brain Research. Developmental Brain Research·C Brodie, A Vernadakis
Jan 1, 1990·Brain Research. Developmental Brain Research·D MangouraA Vernadakis
Dec 18, 1992·Brain Research. Developmental Brain Research·S KentrotiA Vernadakis
Jan 1, 1988·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·D MangouraA Vernadakis
Jan 1, 1991·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·C Brodie, A Vernadakis
Dec 1, 1992·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·D DahlV H Gilad
Aug 1, 1994·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·H RahmanA Vernadakis
Oct 1, 1995·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·N Srivastava, A Vernadakis
Oct 1, 1995·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·P E SpoerriA Vernadakis
Jan 1, 1990·Annals of the New York Academy of Sciences·A VernadakisD Mangoura

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