Growth phase dependency of chromatin cleavage and degradation by bleomycin.

Antimicrobial Agents and Chemotherapy
C W MooreL A Wall

Abstract

Preferential cleavage of Saccharomyces cerevisiae chromosomes in internucleosomal (linker) regions and nonspecific degradation of chromatin by an anticancer antibiotic which degrades DNA were investigated and found to increase in consecutive stages of growth. Cleavage of DNA in internucleosomal regions and intensities and multiplicities of nucleosomal bands were dependent on drug concentration, growth phase of the cells, and length of incubation. Cellular DNA was least degraded during logarithmic phase. After cells progressed only one generation in logarithmic phase, low concentrations (6.7 x 10(-7) to 3.4 x 10(-6) M) of bleomycin produced approximately three to seven times more DNA breaks. Internucleosomal cleavage was highest, and the most extended oligonucleosomal series and extensive chromatin degradation were observed during stationary phase. It is concluded that the growth phase of cells is critical in determining amounts of the highly preferential cleavage in internucleosomal regions and overall breakage and degradation of DNA. Mononucleosomal bands were most intense, indicating the greatest accumulation of DNA of this size. Mean mononucleosomal lengths were 165.9 +/- 3.9 base pairs, in agreement with yeast mononucleosom...Continue Reading

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Citations

Jan 4, 2001·International Journal of Food Microbiology·M S Chae, H Schraft
Oct 26, 2002·Free Radical Biology & Medicine·Darlene DavermannCarol Wood Moore
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Mar 16, 2019·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Teodora TodorovaStephka Chankova

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