Growth-phase-dependent immunodeterminants of Rhizobium trifolii lipopolysaccharide which bind trifoliin A, a white clover lectin.

Journal of Bacteriology
E M HrabakF Dazzo

Abstract

The lipopolysaccharide (LPS) from Rhizobium trifolii 0403 was isolated at different stages of growth and was examined for its (i) ability to bind a white clover lectin (trifoliin A), (ii) immunochemical properties, and (iii) composition. There was significantly more binding of trifoliin A to purified LPS and cells in the early stationary phase than to cells in the exponential phase. Immunofluorescence and enzyme-linked immunosorbent assays indicated that new antigenic determinants of the LPS appeared for brief periods on cells at the end of the lag phase and again at the beginning of the stationary phase. These new antigens were not detected on cells in midexponential or late stationary phase. Monovalent fragments of immunoglobulin G antibodies raised against the unique antigenic determinants in the LPS competitively blocked the binding of trifoliin A to cells in the early stationary phase. Gas chromatographic analysis showed that the relative quantity of several glycosyl components in the LPS increased as the culture advanced from the midexponential to the early stationary phase. In addition, LPS from cells in the early stationary phase had a higher aggregate molecular weight. Quinovosamine (2-amino-2,6-dideoxyglucose) was ide...Continue Reading

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Citations

May 15, 2002·FEMS Microbiology Reviews·Inge Lerouge, Jos Vanderleyden
Mar 24, 2006·Molecular Microbiology·Marc C LausJan W Kijne
Oct 3, 2014·Journal of Immunoassay & Immunochemistry·Olga I GuliyOleg V Ignatov
Jul 25, 2015·PloS One·Soyoun HwangMaor Bar-Peled
Jul 1, 1984·Applied and Environmental Microbiology·G StaceyV Puvanesarajah
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Jul 1, 1984·Applied and Environmental Microbiology·C A Hendrick, L Sequeira
Nov 1, 1986·Applied and Environmental Microbiology·D H Demezas, P J Bottomley
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May 1, 1985·Applied and Environmental Microbiology·K HaahtelaT K Korhonen
May 1, 1985·Applied and Environmental Microbiology·K Haahtela, T K Korhonen
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Jul 1, 1984·Journal of Bacteriology·J E SherwoodG L Truchet
Jul 1, 1984·Analytical Biochemistry·I A van der SchaalJ W Kijne

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