Growth-Phase-Specific Modulation of Cell Morphology and Gene Expression by an Archaeal Histone Protein

MBio
Keely A DulmageAmy K Schmid

Abstract

In all three domains of life, organisms use nonspecific DNA-binding proteins to compact and organize the genome as well as to regulate transcription on a global scale. Histone is the primary eukaryotic nucleoprotein, and its evolutionary roots can be traced to the archaea. However, not all archaea use this protein as the primary DNA-packaging component, raising questions regarding the role of histones in archaeal chromatin function. Here, quantitative phenotyping, transcriptomic, and proteomic assays were performed on deletion and overexpression mutants of the sole histone protein of the hypersaline-adapted haloarchaeal model organism Halobacterium salinarum. This protein is highly conserved among all sequenced haloarchaeal species and maintains hallmark residues required for eukaryotic histone functions. Surprisingly, despite this conservation at the sequence level, unlike in other archaea or eukaryotes, H. salinarum histone is required to regulate cell shape but is not necessary for survival. Genome-wide expression changes in histone deletion strains were global, significant but subtle in terms of fold change, bidirectional, and growth phase dependent. Mass spectrometric proteomic identification of proteins from chromatin enr...Continue Reading

References

Aug 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·M F Mescher, J L Strominger
Nov 1, 1992·Journal of Bacteriology·S TakayanagiM Shioda
Dec 15, 1986·European Journal of Biochemistry·B LaineP Sautiere
Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·G Arents, E N Moudrianakis
Jul 25, 1995·Nucleic Acids Research·A D BaxevanisD Landsman
Jul 22, 1998·Extremophiles : Life Under Extreme Conditions·D SoaresJ N Reeve
Mar 8, 2000·Journal of Molecular Biology·D J SoaresJ N Reeve
Oct 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·W V NgS DasSarma
Sep 22, 2001·Protein Science : a Publication of the Protein Society·R L FahrnerA Slesarev
Dec 26, 2001·Nucleic Acids Research·Ron EdgarAlex E Lash
Oct 2, 2002·Journal of Biomolecular Structure & Dynamics·Nikolai A PavlovAlexei I Slesarev
Mar 5, 2003·BioTechniques·A I SaeedJ Quackenbush
Oct 30, 2003·Nature Structural Biology·Harmit S Malik, Steven Henikoff
Jun 3, 2004·Genome Research·Gavin E CrooksSteven E Brenner
Feb 3, 2005·The Journal of Biological Chemistry·Susumu UchiyamaKiichi Fukui
Feb 4, 2006·Molecular Cell·Richelle SopkoBrenda Andrews
Aug 22, 2006·Current Opinion in Microbiology·Kathleen Sandman, John N Reeve
Jan 11, 2007·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kazuko MatsubaraMasami Horikoshi
Aug 3, 2007·Proceedings of the National Academy of Sciences of the United States of America·Roger D Kornberg
Sep 6, 2007·Genome Research·Amy K SchmidNitin S Baliga
Jul 16, 2008·Nature Structural & Molecular Biology·Shima NakanishiAli Shilatifard
Mar 18, 2010·Journal of Biomolecular Structure & Dynamics·David J Clark
Mar 25, 2010·PloS One·Amber L HartmanJonathan A Eisen
Mar 31, 2010·Proceedings of the National Academy of Sciences of the United States of America·Steven P WilkinsonE Peter Geiduschek
Nov 26, 2010·Nucleic Acids Research·Christina KahramanoglouNicholas M Luscombe
Nov 27, 2010·Nucleic Acids Research·Amy K SchmidNitin S Baliga
Feb 4, 2011·Current Opinion in Microbiology·Sylvie Rimsky, Andrew Travers

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Citations

Dec 19, 2016·The Journal of General and Applied Microbiology·Hiromi Nishida, Taku Oshima
Aug 2, 2017·Proceedings of the National Academy of Sciences of the United States of America·Julie E WalkerThomas J Santangelo
Jun 20, 2018·Journal of Bacteriology·Jonathan H MartinJulie A Maupin-Furlow
Aug 25, 2018·Microbial Genomics·Keely A DulmageAmy K Schmid
Sep 14, 2018·PLoS Genetics·Bram HennemanRemus T Dame
May 4, 2016·Journal of Bacteriology·Alexandra M GehringThomas J Santangelo
Nov 7, 2019·ELife·Maria RojecTobias Warnecke
Jan 1, 2021·Journal of Molecular Biology·Shawn P LaursenKarolin Luger

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Datasets Mentioned

BETA
GSE54599

Methods Mentioned

BETA
PCR
light microscopy
glycosylation

Software Mentioned

DeepView
MODEL
ImageJ
Agilent
PDB
- Viewer
Clustal Omega
arCOG
R package Limma
R

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