Growth Physiology of the Hyperthermophilic Archaeon Thermococcus litoralis: Development of a Sulfur-Free Defined Medium, Characterization of an Exopolysaccharide, and Evidence of Biofilm Formation.

Applied and Environmental Microbiology
K D Rinker, R M Kelly

Abstract

Nutritional characteristics of the hyperthermophilic archaeon Thermococcus litoralis have been investigated with emphasis on the development of a sulfur-free, defined growth medium, analysis of an exocellular polysaccharide, and formation of a biofilm. An artificial-seawater-based medium, containing 16 amino acids, adenine, uracil, vitamins, and trace elements, allowed T. litoralis to attain growth rates and cell densities similar to those found with complex media. Four amino acids (alanine, asparagine, glutamine, and glutamate) were not included due to their lack of effect on growth rates and cell yields. In this medium, cultures reached densities of 10(sup8) cells per ml, with doubling times of 55 min (without maltose) or 43 min (with maltose). Neither the addition of elemental sulfur nor the presence of H(inf2) significantly affected cell growth. A sparingly soluble exopolysaccharide was produced by T. litoralis grown in either defined or complex media. Analysis of the acid-hydrolyzed exopolysaccharide yielded mannose as the only monosaccharidic constituent. This exopolysaccharide is apparently involved in the formation of a biofilm on polycarbonate filters and glass slides, which is inhabited by high levels of T. litoralis....Continue Reading

References

May 1, 1977·Applied and Environmental Microbiology·J E HobbieS Jasper
Feb 1, 1989·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M ItoE De Clercq
Mar 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·S M BarnsN R Pace
Jan 1, 1994·Antonie van Leeuwenhoek·R M Kelly, M W Adams
Mar 1, 1993·Journal of Bacteriology·R N SchichoR M Kelly
Jul 1, 1993·Geochimica Et Cosmochimica Acta·J W Deming, J A Baross
Feb 1, 1985·Applied and Environmental Microbiology·R W RobinsonA S Bleiweis
Jun 1, 1986·Applied and Environmental Microbiology·S BelkinH W Jannasch
May 1, 1988·Applied and Environmental Microbiology·H W JannaschT A Langworthy
Oct 1, 1988·Applied and Environmental Microbiology·J AntónF Rodríguez-Valera
Apr 1, 1992·Applied and Environmental Microbiology·L J SnowdenR M Kelly
Oct 20, 1989·Biotechnology and Bioengineering·B MalikR M Kelly
Apr 5, 1991·Biotechnology and Bioengineering·M A Siebel, W G Characklis
Sep 1, 1975·Microbial Ecology·W N MackA O Ackerson

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Citations

Apr 2, 2008·Annals of the New York Academy of Sciences·Francis E Jenney, Michael W W Adams
Sep 19, 2009·Extremophiles : Life Under Extreme Conditions·Andreas TeskeCarl O Wirsen
Aug 20, 2010·Environmental Technology·Barbara NicolausEbru Toksoy Oner
Oct 19, 2011·Archaea : an International Microbiological Journal·Annarita PoliBarbara Nicolaus
Apr 12, 2012·Journal of Bacteriology·Andrew F GardnerFrancine B Perler
May 3, 2013·PLoS Genetics·Haythem LatifKarsten Zengler
Jul 22, 2020·FEMS Microbiology Letters·James CharlesworthBrendan P Burns
Oct 7, 2004·Applied and Environmental Microbiology·Marybeth A PyszRobert M Kelly
Dec 5, 2012·World Journal of Microbiology & Biotechnology·Hamouda ElabedKamel Gaddour
Dec 5, 2014·Extremophiles : Life Under Extreme Conditions·Derrick L LewisRobert M Kelly
Dec 21, 2012·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·François OrangeDaniel Prieur
Jun 7, 2015·Applied and Environmental Microbiology·Haythem LatifKarsten Zengler
Sep 26, 2015·Applied Microbiology and Biotechnology·Giuseppe SquillaciAlessandra Morana
Jul 19, 2008·Biotechnology and Bioengineering·V MuralidharanR M Kelly
May 17, 2011·Nature Reviews. Microbiology·Sonja-Verena Albers, Benjamin H Meyer
Jan 30, 2013·Biochemical Society Transactions·Sabrina Fröls
Jul 3, 2013·Annual Review of Microbiology·Alvaro OrellSonja-Verena Albers
Jan 18, 2017·Archives of Microbiology·Preeti Ranawat, Seema Rawat
Apr 2, 2008·Annals of the New York Academy of Sciences·Amy L VanfossenRobert M Kelly
Oct 25, 2016·Environmental Microbiology Reports·Man-Young JungSung-Keun Rhee
Dec 1, 1998·Journal of Applied Microbiology·M W Adams
Jul 5, 2012·Nature Communications·Joshua A LermanBernhard O Palsson
Oct 1, 2017·AMB Express·Anna LewinMark R Liles
Nov 29, 2017·Applied and Environmental Microbiology·Consolazione CarusoAngelina Lo Giudice
Dec 15, 2017·Environmental Science and Pollution Research International·Preeti Ranawat, Seema Rawat
Aug 12, 2018·Nature Reviews. Microbiology·Marleen van WolferenSonja-Verena Albers
Jan 22, 2021·FEMS Microbiology Reviews·April M LewisRobert M Kelly
Feb 21, 2021·International Journal of Biological Macromolecules·Mayra Alejandra López-OrtegaAdriana Inés Rodríguez-Hernández

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