PMID: 7543481Aug 1, 1995Paper

Growth properties associated with A-U replacement of specific G-C base pairs in 16S rRNA from Escherichia coli

Journal of Bacteriology
K L Triman

Abstract

Mutations that disrupt each of seven specific G-C base pairs in 16S rRNA from Escherichia coli confer loss of expression of a plasmid-encoded 16S rRNA selectable marker (spectinomycin resistance). However, A-U replacement of G-C base pairs at nucleotides 359/52 or 1292/1245 in 16S rRNA permits normal expression of the marker. By contrast, A-U replacements at 146/176, 153/168, 350/339, or 1293/1244 are associated with loss of expression of the marker. These genetic studies are designed to determine the importance of specific base pairs by assessment of the structural and functional impairments of 16S rRNA molecules resulting from expression of base pair substitutions at these positions.

References

May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M SanterJ McLaughlin
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T Powers, H F Noller
Oct 20, 1989·Journal of Molecular Biology·K TrimanH F Noller
Jan 1, 1988·Annals of the New York Academy of Sciences·L B Evnin, C S Craik
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel

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