Growth Rate and Biofilm Formation Ability of Clinical and Laboratory-Evolved Colistin-Resistant Strains of Acinetobacter baumannii

Frontiers in Microbiology
Zahra FarshadzadehAbbas Bahador

Abstract

Two different mechanisms of resistance to colistin in Acinetobacter baumannii have been described. The first involves the total loss of lipopolysaccharide (LPS) due to mutations in the lpxACD operon, which is involved in the lipid A biosynthesis pathway. The second entails the addition of ethanolamine to the lipid A of the LPS resulting from mutations in the PmrAB two-component system. To evaluate the impact of colistin resistance-associated mutations on antimicrobial resistance and virulence properties, four pairs of clinical and laboratory-evolved colistin-susceptible/colistin-resistant (ColS/ColR) A. baumannii isolates were used. Antimicrobial susceptibility, surface motility, in vitro and in vivo biofilm-forming capacity, in vitro and in vivo expression levels of biofilm-associated genes, and in vitro growth rate were analyzed in these strains. Growth rate, in vitro and in vivo biofilm formation ability, as well as expression levels of biofilm-associated gene were reduced in ColR LPS-deficient isolate (the lpxD mutant) when compared with its ColS partner, whereas there were not such differences between LPS-modified isolates (the pmrB mutants) and their parental isolates. Mutation in lpxD was accompanied by a greater reducti...Continue Reading

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Citations

Jan 19, 2019·Pathogens and Disease·Fei-Ju LiGaetan Burgio
Nov 22, 2018·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Yaakov DicksteinUNKNOWN AIDA Study Group
Jul 3, 2021·Microorganisms·Arianna PompilioCecilia Ambrosi

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Methods Mentioned

BETA
PCR
Assay
motility assay
glycosylation

Software Mentioned

BLAST
Primer3web
Basic Local Alignment Search Tool ( BLAST )

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