Growth rate and myofibroblast differentiation of desmoid fibroblast-like cells are modulated by TGF-β signaling

Histochemistry and Cell Biology
Maria Vittoria EnzoDaniela Segat

Abstract

Desmoid-like fibromatosis (DF) is a rare myofibroblastic benign tumor, often associated with local and repeated injuries, spontaneous regression and stabilization of disease progression suggesting the involvement of altered Wnt/β-catenin signaling activation and/or aberrant response of the DF cells to external environmental stimuli. The aim of this study was to investigate the response of DF cells to microenvironmental factors such as inflammatory and growth factors or hormones. We observed that the inflammatory cytokine, transforming growth factor-β (TGF-β1) stimulated cell growth and myofibroblast differentiation of DF cells regardless of the presence of a β-catenin mutation. The role of TGF-β1 in cell growth and myogenic differentiation of in vitro cultures of primary DF cells and normal fibroblasts was investigated by gene and protein expression analyses. We demonstrated that TGF-β1 exerted its role via the canonical Smad pathway with the phosphorylation of Smad3 being crucial for TGF-β1 dependent DF cell growth and myofibroblastic differentiation. Furthermore we demonstrated that cell confluence is a critical determinant of TGF-β1 inducing the DF myofibroblast differentiation, implying that the intercellular communications...Continue Reading

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Citations

Sep 25, 2020·European Journal of Radiology·Milea J M TimbergenJacob J Visser
Jan 21, 2020·Bulletin du cancer·Sébastien Salas, Frédéric Chibon

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Methods Mentioned

BETA
biopsies
biopsy
enzyme-linked immunosorbent assay
ELISA
protein assay

Software Mentioned

AmpliSeq
ZEN
Image Lab
MACPROBE
TMAP ( Torrent Mapping Alignment Program )
LSM

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