Growth Rate-Dependent Modulation of Carbon Flux through Central Metabolism and the Kinetic Consequences for Glucose-Limited Chemostat Cultures of Corynebacterium glutamicum.

Applied and Environmental Microbiology
Muriel Cocaign-BousquetNic D Lindley

Abstract

The physiological behavior of Corynebacterium glutamicum in glucose-limited chemostat cultures was examined from both growth kinetics and enzymatic viewpoints. Metabolic fluxes within the central metabolism were calculated from growth kinetics and analyzed in relation to specific enzyme activities. At high growth rates, incomplete glucose removal was observed, and this was attributed to rate-limiting capacity of the phosphotransferase system transporter and the probable contribution of a low-affinity permease uptake mechanism. The improved biomass yield observed at high growth rates was related to a shift in the profile of anaplerotic carboxylation reactions, with pyruvate carboxylase replacing malic enzyme. Phosphoenolpyruvate carboxylase, an activity often assumed to be the major anaplerotic reaction during growth of C. glutamicum on glucose, was present at only low levels and is unlikely to contribute significantly to tricarboxylic acid cycle fuelling other than at low growth rates.

References

Jan 15, 1995·European Journal of Biochemistry·C RollinJ L Guerquin-Kern
Mar 15, 1993·Biotechnology and Bioengineering·J J Vallino, G Stephanopoulos
Aug 1, 1990·Trends in Ecology & Evolution·D E Dykhuizen, A M Dean

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Citations

Oct 9, 2004·Biotechnology and Bioengineering·Nina GunnarssonJens Nielsen
Apr 12, 2011·Applied and Environmental Microbiology·Steffen N LindnerVolker F Wendisch
Jun 27, 2019·Applied Microbiology and Biotechnology·Maike KortmannMichael Bott
Jul 6, 2000·Applied and Environmental Microbiology·P GourdonA Guyonvarch
Dec 22, 2006·Journal of Molecular Microbiology and Biotechnology·Min-Woo MoonJung-Kee Lee
Apr 1, 2009·Microbiology·Christiane DietrichArmel Guyonvarch
Aug 10, 1999·Biotechnology and Bioengineering·A L Santerre HenriksenJ Nielsen
Nov 6, 2008·Biotechnology and Bioengineering·Kjeld Raunkjaer Kjeldsen, Jens Nielsen
Aug 17, 2005·FEMS Microbiology Reviews·Uwe Sauer, Bernhard J Eikmanns
Jul 4, 2012·International Journal of Molecular Sciences·Mohsen ZareianNazamid Saari
Apr 3, 2019·Journal of Industrial Microbiology & Biotechnology·Jian-Zhong XuWei-Guo Zhang
Aug 11, 2020·Engineering in Life Sciences·Roman P JansenStephan Noack
Nov 15, 2000·Biological Chemistry·H SahmA A de Graaf
Nov 13, 2020·Frontiers in Bioengineering and Biotechnology·Michaela GrafRalf Takors
Feb 9, 2021·Frontiers in Bioengineering and Biotechnology·Jannick KappelmannStephan Noack
Apr 1, 1997·Microbiology·Petra G Peters-WendischHermann Sahm
Aug 11, 2000·Metabolic Engineering·P Gourdon, N D Lindley
Feb 16, 2000·Journal of Biotechnology·J C PortaisJ N Barbotin
Jul 1, 1997·Applied and Environmental Microbiology·F AmpeN D Lindley

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