Growth rates of protein crystals.

Journal of the American Chemical Society
Jeremy D Schmit, Ken A Dill

Abstract

Protein crystallization is important for structural biology. The rate at which a protein crystallizes is often the bottleneck in determining the protein's structure. Here, we give a physical model for the growth rates of protein crystals. Most materials crystallize faster under stronger growth conditions; however, protein crystallization slows down under the strongest conditions. Proteins require a crystallization slot of 'just right' conditions. Our model provides an explanation. Unlike simpler materials, proteins are orientationally asymmetrical. Under strong conditions, protein molecules attempt to crystallize too quickly, in wrong orientations, blocking surface sites for more productive crystal growth. The model explains the observation that increasing the net charge on a protein increases the crystal growth rate. The model predictions are in good agreement with experiments on the growth rates of tetragonal lysozyme crystals as a function of pH, salt concentration, temperature, and protein concentration.

References

Apr 27, 1999·Acta Crystallographica. Section D, Biological Crystallography·J R Luft, G T DeTitta
Jun 19, 2001·Biophysical Chemistry·A M Kierzek, P Zielenkiewicz
Nov 10, 2001·Annual Review of Biomedical Engineering·J M Wiencek
Jul 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·A George, W W Wilson
Jan 30, 2009·Protein Science : a Publication of the Protein Society·Gerald R GrimsleyC Nick Pace
Mar 5, 2010·The Journal of Physical Chemistry. B·Jeremy D Schmit, Ken A Dill
Sep 8, 2011·The Journal of Chemical Physics·Jeremy D SchmitKen Dill

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Citations

Jul 9, 2013·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Chen Yan ZhangPeng Shang
Feb 16, 2016·The Journal of Chemical Physics·Stephen WhitelamJeremy D Schmit
Aug 4, 2015·Colloids and Surfaces. B, Biointerfaces·Diana Fusco, Patrick Charbonneau
Jun 12, 2013·International Journal of Molecular Sciences·Chen-Yan ZhangDa-Chuan Yin
Jul 30, 2018·Protein Science : a Publication of the Protein Society·Renee J AriasDouglas C Rees
Jul 10, 2014·Soft Matter·Lester O HedgesStephen Whitelam
Oct 3, 2014·Physical Chemistry Chemical Physics : PCCP·C LabbezM Delhorme
Nov 28, 2019·Physical Review. E·Tien M PhanJeremy D Schmit
Mar 1, 2018·The Journal of Physical Chemistry. B·Caleb HuangJeremy D Schmit

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