PMID: 6991126Feb 1, 1980Paper

Growth regulation of lens epithelial cells. Chemically-modified sepharose as a suitable substratum for studying cell-substratum interactions

Cell Differentiation
M IwigD Glaesser

Abstract

Suitable substrata for studying cell-substratum interactions were prepared by melting of unsubstituted and substituted agarose beads (Sepharose). The reductive alkylation procedure proved to be useful in introducing various ligands into the gel matrix. The resulting bonds remained stable during sterilisation and melting. The introduction of charges into the gel matrix facilitated cell adhesion to the substratum. But only positively-charged agarose substrata were as effective as glass surfaces in supporting cell attachment. The correlation found between cell attachment and the stimulation of DNA synthesis demonstrates that lens epithelial cells are strongly anchorage dependent.

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Citations

Apr 5, 1994·Biotechnology and Bioengineering·R SinghviD I Wang
Jul 1, 1981·Molecular and Cellular Endocrinology·B TeyssotL M Houdebine
Aug 12, 1999·Acta histochemica·V H Latham, S B Oppenheimer
Aug 12, 1999·Acta histochemica·B A SalbillaS B Oppenheimer
Jan 1, 1981·Differentiation; Research in Biological Diversity·J Piatigorsky
Oct 1, 1986·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·R G MacBride, R J Przybylski
Jan 1, 1983·Annals of the New York Academy of Sciences·S SegalM Feldman

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