PMID: 701380Oct 1, 1978Paper

Growth-related fluctuation in messenger RNA utilization in animal cells

The Journal of Cell Biology
G T Lee, D L Engelhardt

Abstract

Monkey fibroblasts maintained in culture regulate their levels of intracellular protein throughout the growth cycle by means of variations in the rate of protein biosynthesis. Cytoplasmic mRNA in stationary phase cells was compared to that in exponential phase cells. In stationary phase cells 56% of the cytoplasmic polyadenylated RNA was found in the 40--90S postpolysomal region of sucrose sedimentation gradients, while only 23% was found in this region in exponential phase cells. Analysis of electron micrographs of sectioned exponential and stationary phase cells revealed that this shift in polyadenylated RNA location is accompanied by a loss of polysome-like aggregates of ribosomes. Most if not all of this species of postpolysomal polyadenylated RNA is not being translated by single ribosomes since no detectable amounts of nascent peptide were present in this region. This nonpolysomal polyadenylated RNA is comparable in size to polysomal polyadenylated RNA. The length of the 3'-poly(A) tract was also comparable for these two species. The extent of capping of poly(A)-containing molecules was also comparable for these two species. The template activity of nonpolysomal RNA in a wheat germ extract was comparable to that of polyso...Continue Reading

References

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Citations

Feb 1, 1996·Molecular Reproduction and Development·K C Kleene
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G ThomasH Luther
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R YenofskyG Brawerman
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S B Horowitz, L J Tluczek
Feb 15, 1984·European Journal of Biochemistry·L GalegoC Rodrigues-Pousada
Apr 1, 1983·Kidney International·A J Ouellette
Nov 15, 1984·European Journal of Biochemistry·L I Slobin, P Jordan
Jan 29, 1985·Biochimica Et Biophysica Acta·M M SalimansH O Voorma
May 5, 1980·Journal of Molecular Biology·A J Minty, F Gros
May 15, 1986·Archives of Biochemistry and Biophysics·J L NgoK H Ibsen
Apr 5, 1979·Journal of Molecular Biology·G T Lee, D L Engelhardt

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