Growth-related Metabolism of the Carbon Storage Poly-3-hydroxybutyrate in Legionella pneumophila.

The Journal of Biological Chemistry
Nadine GillmaierKlaus Heuner

Abstract

Legionella pneumophila, the causative agent of Legionnaires disease, has a biphasic life cycle with a switch from a replicative to a transmissive phenotype. During the replicative phase, the bacteria grow within host cells in Legionella-containing vacuoles. During the transmissive phenotype and the postexponential (PE) growth phase, the pathogens express virulence factors, become flagellated, and leave the Legionella-containing vacuoles. Using (13)C labeling experiments, we now show that, under in vitro conditions, serine is mainly metabolized during the replicative phase for the biosynthesis of some amino acids and for energy generation. During the PE phase, these carbon fluxes are reduced, and glucose also serves as an additional carbon substrate to feed the biosynthesis of poly-3-hydroxybuyrate (PHB), an essential carbon source for transmissive L. pneumophila. Whole-cell FTIR analysis and comparative isotopologue profiling further reveal that a putative 3-ketothiolase (Lpp1788) and a PHB polymerase (Lpp0650), but not enzymes of the crotonyl-CoA pathway (Lpp0931-0933) are involved in PHB metabolism during the PE phase. However, the data also reflect that additional bypassing reactions for PHB synthesis exist in agreement with...Continue Reading

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Citations

Jul 28, 2016·FEBS Letters·Wolfgang Eisenreich, Klaus Heuner
Dec 21, 2016·Molecular Microbiology·Adrian MehlitzThomas Rudel
Feb 7, 2018·Frontiers in Cellular and Infection Microbiology·Giulia OlivaCarmen Buchrieser
Jun 9, 2017·MSystems·Lena KönigMatthias Horn
Jan 6, 2019·Applied and Environmental Microbiology·Luis Paulo Silveira AlvesGary Stacey
Nov 7, 2017·Frontiers in Cellular and Infection Microbiology·Sandra Appelt, Klaus Heuner
Jul 7, 2017·Frontiers in Cellular and Infection Microbiology·Fan ChenKlaus Heuner
May 31, 2018·Scientific Reports·Ashley BestYousef Abu Kwaik
Jul 15, 2017·Frontiers in Cellular and Infection Microbiology·Ina HäusleinWolfgang Eisenreich
Apr 25, 2020·International Journal of Molecular Sciences·Justyna MierziakJan Szopa
Aug 28, 2019·International Journal of Medical Microbiology : IJMM·Kristin KöppenKlaus Heuner
Feb 2, 2021·Frontiers in Cellular and Infection Microbiology·Wolfgang EisenreichWerner Goebel
May 24, 2020·International Journal of Medical Microbiology : IJMM·Fan ChenKlaus Heuner
Apr 28, 2021·International Journal of Medical Microbiology : IJMM·Mareike KunzeWolfgang Eisenreich

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