Growth requirements of a new food-vacuole-less mutant of Tetrahymena

European Journal of Protistology
A TiedtkeL Rasmussen

Abstract

The growth requirements of the ciliate Tetrahymena thermophila have been known for a long time. In this study we have investigated the additional requirements induced in a cell line defective in phagocytosis. The mutant cell line of T. thermophila, II 8 G, derived from the wildtype, T. thermophila, CU 399, has apparently no oral structures and forms no food vacuoles at 37°C, but has normal oral structures and the ability to form food vacuoles at normal rates at 28°C. Taking advantage of a synthetic nutrient medium, we observed that the mutant cell line shows increased requirement for Fe(III), Cu(++) and folic acid under restrictive conditions. Phagocytosis obviously plays a role in utilization of low concentrations of the three substances, whereas all other required substances can enter fast enough to support short generation times.

References

Feb 1, 1975·Experimental Cell Research·E Orias, N A Pollock

Related Concepts

Ciliata
Copper
Curcumin
Folic Acid
Food
Phagocytosis
Cell Line, Tumor
Oral Cavity
Mutant
Pseudonocardia thermophila

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