PMID: 9649922Jul 3, 1998Paper

Growth response of human coronary smooth muscle cells to angiotensin II and influence of angiotensin AT1 receptor blockade

Coronary Artery Disease
S HafiziM H Yacoub

Abstract

The renin-angiotensin system has been implicated in the development of vascular wall thickening in cardiovascular disease, through the growth-promoting actions of the vasoconstrictive agent, angiotensin II, on vascular smooth muscle cells. To investigate the effect of angiotensin II on growth of human coronary artery smooth muscle cells (cSMCs) in culture, and to identify the angiotensin receptor(s) mediating such a response. Human cSMCs were isolated from coronary arteries of recipient hearts obtained during transplantation, and characterized by immunohistochemistry. The effect of angiotensin II on protein synthesis by cSMCs was measured by [3H]leucine incorporation and protein concentration assays. Human cSMC proliferation was assessed by [3H]thymidine incorporation assay and cell count. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect angiotensin receptor expression. Transient increases in intracellular calcium concentration in cSMCs in response to angiotensin II stimulation were visualized under fura-2 fluorescence microscopy. Angiotensin II (1 nmol/l-10 mumol/l) stimulated protein synthesis in cSMCs (maximum 24 +/- 2% increase in incorporation of [3H]leucine over 48 h; n = 4, P < 0.01). An increa...Continue Reading

Citations

Aug 3, 2005·International Journal of Cardiology·L J WagenaarW H van Gilst
Jun 9, 2004·Biochemical and Biophysical Research Communications·Jonas StenhoffSassan Hafizi
Apr 23, 2002·Journal of the Renin-angiotensin-aldosterone System : JRAAS·A D Hughes
Apr 8, 2006·Circulation Research·Sergey PryshchepCornelia M Weyand
Feb 11, 2005·American Journal of Physiology. Renal Physiology·Aihua ZhangTianxin Yang
Aug 20, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Sassan HafiziChristopher G Proud

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