Abstract
The renin-angiotensin system has been implicated in the development of vascular wall thickening in cardiovascular disease, through the growth-promoting actions of the vasoconstrictive agent, angiotensin II, on vascular smooth muscle cells. To investigate the effect of angiotensin II on growth of human coronary artery smooth muscle cells (cSMCs) in culture, and to identify the angiotensin receptor(s) mediating such a response. Human cSMCs were isolated from coronary arteries of recipient hearts obtained during transplantation, and characterized by immunohistochemistry. The effect of angiotensin II on protein synthesis by cSMCs was measured by [3H]leucine incorporation and protein concentration assays. Human cSMC proliferation was assessed by [3H]thymidine incorporation assay and cell count. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect angiotensin receptor expression. Transient increases in intracellular calcium concentration in cSMCs in response to angiotensin II stimulation were visualized under fura-2 fluorescence microscopy. Angiotensin II (1 nmol/l-10 mumol/l) stimulated protein synthesis in cSMCs (maximum 24 +/- 2% increase in incorporation of [3H]leucine over 48 h; n = 4, P < 0.01). An increa...Continue Reading
Citations
Aug 3, 2005·International Journal of Cardiology·L J WagenaarW H van Gilst
Oct 26, 1999·Atherosclerosis·S HafiziM H Yacoub
Oct 18, 2002·Peptides·Atsuo TaharaAkihiro Tanaka
Jun 9, 2004·Biochemical and Biophysical Research Communications·Jonas StenhoffSassan Hafizi
Dec 21, 2004·Vascular Pharmacology·Sassan HafiziMagdi H Yacoub
Nov 24, 2004·Neuroscience Letters·Zhongbiao WangWalter H Newman
Mar 11, 2000·Acta Physiologica Scandinavica·M HouL Edvinsson
Nov 7, 1998·Acta Physiologica Scandinavica·S HafiziM H Yacoub
Jul 16, 1999·Clinical and Experimental Pharmacology & Physiology·S HafiziM H Yacoub
Nov 27, 2001·British Journal of Cancer·E RiveraJ Sotelo
Apr 23, 2002·Journal of the Renin-angiotensin-aldosterone System : JRAAS·A D Hughes
Apr 8, 2006·Circulation Research·Sergey PryshchepCornelia M Weyand
Nov 18, 2000·Hypertension·J IbrahimP S Sever
Dec 8, 1998·Circulation·S HafiziM H Yacoub
Mar 9, 2002·Circulation Research·Stephen ScottMartin R Bennett
Feb 11, 2005·American Journal of Physiology. Renal Physiology·Aihua ZhangTianxin Yang
Jun 14, 2001·Journal of Hypertension·T WatanabeC R Benedict
May 17, 2013·Endocrinology·Almudena Gómez-HernándezManuel Benito
Aug 20, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Sassan HafiziChristopher G Proud
Sep 29, 2017·Frontiers in Medicine·Rafia S Al-LamkiJordan S Pober