PMID: 6298788Mar 1, 1983Paper

Growth stimulation of A431 cells by epidermal growth factor: identification of high-affinity receptors for epidermal growth factor by an anti-receptor monoclonal antibody

Proceedings of the National Academy of Sciences of the United States of America
T KawamotoJ Mendelsohn

Abstract

Epidermal growth factor (EGF) at 3 nM maximally inhibits the proliferation of A431 epidermoid carcinoma cells. We show that at lower concentrations, in the range of 3-100 pM, EGF has a mitogenic effect on A431 cells. In the presence of 100 nM anti-EGF-receptor monoclonal IgG (designated 528), which inhibits A431 cell proliferation and blocks greater than 95% of EGF binding, EGF becomes mitogenic for A431 cells at concentrations up to 3 nM. These results suggest that a minor population of high-affinity EGF receptors may be involved in stimulation of A431 cell proliferation. Saturation binding assays with 125I-labeled EGF indicate that approximately equal to 0.1-0.2% of receptors for EGF are high-affinity receptors that bind EGF with an estimated Kd of 7 X 10(-11) M. This affinity is nearly 2 orders of magnitude higher than that of the remaining EGF receptors. Although A431 cell proliferation is maximally inhibited by nonsaturating amounts of EGF (3 nM), maximal inhibition by 528 IgG (approximately equal to 70% of maximal inhibition by EGF) requires saturating concentrations of antibody (approximately equal to 15 nM). Unlike EGF, rapid down-regulation is not observed with 528 IgG. These results indicate different mechanisms of gr...Continue Reading

References

Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·F R MaxfieldI Pastan
Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·Y ShechterP Cuatrecasas
Jun 21, 1979·Nature·J L GoldsteinM S Brown
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R N FabricantG J Todaro
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M N Krupp, J N Livingston
Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J M Maturo, M D Hollenberg
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M Das, C F Fox
Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·H HaiglerS Cohen
Jan 1, 1979·Annual Review of Biochemistry·G Carpenter, S Cohen
Jun 1, 1976·Journal of Cellular Physiology·G Carpenter, S Cohen
Dec 16, 1980·Biochemical and Biophysical Research Communications·A C KingP Cuatrecasas
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·A B SchreiberJ Schlessinger
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J E BussG N Gill
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G E Landreth, E M Shooter
Mar 1, 1980·Analytical Biochemistry·D Barnes, G Sato
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H MurakamiT Kano-Sueoka

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Citations

May 1, 1996·Journal of Cellular Physiology·M I Garcia-LloretL J Guilbert
Jul 1, 1990·Journal of Cellular Physiology·A Van der MeerenM C Jaurand
Sep 1, 1993·Journal of Cellular Physiology·R L Konger, T C Chan
Sep 28, 2002·Journal of Magnetic Resonance Imaging : JMRI·Francis G Blankenberg, H William Strauss
Jan 1, 1990·Archives of Dermatological Research·G ZambrunoA Giannetti
Jan 1, 1994·Breast Cancer Research and Treatment·J Baselga, J Mendelsohn
Jan 1, 1995·Cancer Chemotherapy and Pharmacology·V K Khetarpal, L S Storbeck
Jan 1, 1991·Cancer Immunology, Immunotherapy : CII·R PellegriniM I Colnaghi
Jan 1, 1991·Cancer Immunology, Immunotherapy : CII·S H TorpG Unsgaard
Dec 17, 1993·Pharmacy World & Science : PWS·R K Sweeb, J H Beijnen
Nov 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M Rosdy
Oct 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·D BarnesG H Sato
May 1, 1986·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M RosdyM Darmon
Aug 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M PonecJ Boonstra
Sep 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·Y MasudaK Nishikawa
May 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·Y MyokenJ D Sato
Jun 1, 2010·In Vitro Cellular & Developmental Biology. Animal·Gordon H SatoDavid W Barnes
Oct 12, 2011·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Agnieszka DreierJoachim Weis

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