Growth suppression and apoptosis-like cell death in Microcystis aeruginosa by H2 O2 : A new insight into extracellular and intracellular damage pathways

Chemosphere
Tingru ZhouYi Tao

Abstract

H2O2 has been suggested and applied as effective algaecide for harmful cyanobacterial bloom control, however, the transport of exogenous H2O2 into microalgal cells, the subsequent intracellular damage pathway and dose-response variations were little studied. We addressed these questions in a bloom-forming cyanobacterium Microcystis aeruginosa with H2O2 at 0.1-1.5 mM. The results showed that H2O2 at 0.4 mM and above significantly suppressed M. aeruginosa growth for over two weeks, and induced apoptosis-like death in terms of membrane potential dissipation, caspase-3 activation, chromatin condensation, and lysis induction. However, the dose-response effects were not monotonic. H2O2 at 0.7 mM resulted in the severest growth suppression among 0.1-1.5 mM treatments, including the lowest biomass for 74% loss, the highest cell lysis ratio for 79%, and the highest utilization rate of H2O2 for 0.101 mM d-1. Moreover, several evidence point to severer apoptosis-like cell death in 0.7 mM treatments, involving fastest and severest cell lysis, smallest cell size and wrinkled surface and lowest membrane potential. Therefore, the apoptosis-like cell death induced by H2O2 at moderate dosages should be a crucial cause for the non-monotonic dose...Continue Reading

Citations

Jul 14, 2020·Journal of Hazardous Materials·Xiaoqin ZhuHongying Hu
Mar 23, 2021·Frontiers in Microbiology·Anabella AguileraMaría Victoria Martin
Apr 17, 2021·Environmental Science and Pollution Research International·Lixiao NiXuqing Chen
Jul 30, 2021·The Science of the Total Environment·Binliang WangKongxian Zhu
Aug 17, 2021·Frontiers in Microbiology·Leda GiannuzziGuillermo Blanco

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