GRP78 Promotes Neural Stem Cell Antiapoptosis and Survival in Response to Oxygen-Glucose Deprivation (OGD)/Reoxygenation through PI3K/Akt, ERK1/2, and NF-κ B/p65 Pathways

Oxidative Medicine and Cellular Longevity
Qian LiuXinfeng Liu

Abstract

When brain injury happens, endogenous neural stem cells (NSCs) located in the adult subventricular zone (SVZ) and subgranular zone (SGZ) are attacked by ischemia/reperfusion to undergo cellular apoptosis and death before being induced to migrate to the lesion point and differentiate into mature neural cells for damaged cell replacement. Although promoting antiapoptosis and NSC survival are critical to neuroregeneration, the mechanism has yet been elucidated clearly. Here in this study, we established an in vitro oxygen-glucose deprivation (OGD)/reoxygenation model on NSCs and detected glucose-regulated protein 78 (GRP78) involved in apoptosis, while in the absence of GRP78 by siRNA transfection, OGD/reoxygenation triggered PI3K/Akt, ERK1/2, and NF-κB/p65 activation, and induced NSC apoptosis was attenuated. Further investigation, respectively, with the inhibitor of PI3K/Akt or ERK1/2 demonstrated a blockage on GRP78 upregulation, while the inhibition of NF-κB rarely affected GRP78 induction by OGD/reoxygenation. The results indicated the bidirectional regulations of GRP78-PI3K/Akt and GRP78-ERK1/2 and the one-way signalling transduction through GRP78 to NF-κB/p65 on NSC survival from OGD/reoxygenation. In conclusion, we found t...Continue Reading

References

Jun 2, 2000·Science·D L ClarkeJ Frisén
Aug 10, 2000·The Journal of Biological Chemistry·R M Seiser, C V Nicchitta
Jan 10, 2001·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·P H Chan
Oct 16, 2004·Science·Umut OzcanGökhan S Hotamisligil
Oct 4, 2005·The Journal of Clinical Investigation·Chunyan XuJohn C Reed
Aug 12, 2006·Cell·Yoko ShibataTom A Rapoport
Sep 29, 2009·Clinical Science·Toru Hosoi, Koichiro Ozawa
Apr 19, 2012·Biotechnology and Bioengineering·Lars KoberJuergen Bode
May 15, 2012·Haematologica·Lauren I Aronson, Faith E Davies
Jan 21, 2014·Biochimica Et Biophysica Acta·Nicola J Darling, Simon J Cook
Apr 4, 2014·Biological & Pharmaceutical Bulletin·Kazushi MatsumuraMitsuru Hashida
Feb 24, 2015·Frontiers in Cellular Neuroscience·Vanessa M MachadoInês M Araújo
Apr 30, 2015·Glia·Catherine-Alexandra GrégoireKarl J L Fernandes
May 6, 2015·Frontiers in Oncology·Hironori Kato, Hideki Nishitoh
Sep 16, 2015·International Journal of Molecular Sciences·Zhangyuan XiongFengjin Guo
Aug 5, 2016·Journal of Stroke·Zhenggang Zhang, Michael Chopp
Apr 21, 2017·Frontiers in Neuroscience·Caty Casas
Jul 22, 2017·Nature Reviews. Neurology·Claudio Hetz, Smita Saxena
Aug 5, 2017·Cell and Tissue Research·Rebecca M Ruddy, Cindi M Morshead
Sep 15, 2017·Journal of Biochemistry·Naoya Murao, Hideki Nishitoh

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Methods Mentioned

BETA
protein folding
transfection
electrophoresis
X-ray
ELISA
transfecting
nuclear translocation
nuclear

Software Mentioned

ImageJ

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