GSK-3 activity is critical for the orientation of the cortical microtubules and the dorsoventral axis determination in zebrafish embryos.

PloS One
Ming ShaoHongwei Zhang

Abstract

The formation of dorsal-ventral (D-V) axis is the earliest event that breaks the radial symmetry and determines the bilateral body plan of a vertebrate embryo, however, the maternal control of this process is not fully understood. Here, we discovered a new dorsalizing window of acute lithium treatment, which covers only less than 10 minutes after fertilization. Lithium treatment in this window was not able to reverse the ventralized phenotype in tokkeabi (tkk) mutant embryos, and its dorsalizing activity on wild-type embryos was inhibited by nocodazole co-treatment. These evidences indicate that the underlying mechanism is independent of a direct activation of Wnt/β-catenin signaling, but depends on the upstream level of the microtubule mediated dorsal determinant transport. In order to identify the target of lithium in this newly discovered sensitive window, GSK-3 inhibitor IX as well as the IMPase inhibitor L690, 330 treatments were performed. We found that only GSK-3 inhibitor IX treatment mimicked the lithium treatment in the dorsalizing activity. Further study showed that the parallel pattern of cortical microtubules in the vegetal pole region and the directed migration of the Wnt8a mRNA were randomized by either lithium o...Continue Reading

References

Jul 1, 1989·Developmental Biology·S R ScharfJ C Gerhart
Mar 1, 1989·Developmental Biology·K R Kao, R P Elinson
Jan 1, 1993·Brain Research Bulletin·J A Pineda, M Westerfield
Jul 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C B KimmelT F Schilling
Oct 1, 1995·Current Opinion in Genetics & Development·W Driever
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·P S Klein, D A Melton
Mar 1, 1996·Mechanisms of Development·J JooreD Zivkovic
Jan 1, 1997·Current Biology : CB·S Jesuthasan, U Stähle
Jan 1, 1997·Annual Review of Cell and Developmental Biology·R Harland, J Gerhart
Jan 8, 1999·Cell Biology International·G SconzoG Giudice
Sep 1, 2000·Brain Research. Brain Research Reviews·L BuéeP R Hof
Feb 13, 2001·Biochemical and Biophysical Research Communications·W J Ryves, A J Harwood
Jun 1, 2001·Progress in Neuro-psychopharmacology & Biological Psychiatry·A ShaldubinaR H Belmaker
Sep 6, 2002·Journal of Alzheimer's Disease : JAD·G V Johnson, J A Hartigan
Oct 2, 2002·Results and Problems in Cell Differentiation·Masahiko HibiIgor B Dawid
Nov 21, 2002·Pharmacology & Therapeutics·Nadia Gurvich, Peter S Klein
Jul 23, 2003·Developmental Biology·Trudi A WestfallDiane C Slusarski
Apr 14, 2005·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Todd D Gould, Husseini K Manji
Nov 16, 2005·Annual Review of Genetics·Alexander F Schier, William S Talbot
Feb 17, 2006·Nature Reviews. Molecular Cell Biology·Edward M De Robertis
Mar 30, 2006·Proceedings of the National Academy of Sciences of the United States of America·Federico Coluccio LeskowMarcelo G Kazanietz
Feb 24, 2007·Current Alzheimer Research·Ira Driscoll, Susan M Resnick
Sep 8, 2009·Mechanisms of Development·E M De Robertis
Sep 14, 2011·Proceedings of the National Academy of Sciences of the United States of America·Fu-I LuBernard Thisse

❮ Previous
Next ❯

Methods Mentioned

BETA
confocal microscopy
transgenic
in vitro transcription

Related Concepts

Related Feeds

Bipolar Disorder

Bipolar disorder is characterized by manic and/or depressive episodes and associated with uncommon shifts in mood, activity levels, and energy. Discover the latest research this illness here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Antipsychotic Drugs

Antipsychotic drugs are a class of medication primarily used to manage psychosis (including delusions, hallucinations, paranoia or disordered thought), principally in schizophrenia and bipolar disorder. Discover the latest research on antipsychotic drugs here

Axis Formation

Three major axes, anteroposterior, dorsoventral, and left-right axes, patterned during embryogenesis determine the overall body plan of an organism. Discover the latest research on axis formation here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.