GSK-3 inhibitor inhibits cell proliferation and induces apoptosis in human osteosarcoma cells

Oncology Reports
Hideki NishimuraTetsuji Yamamoto

Abstract

Glycogen synthase kinase-3 (GSK-3) is a serine/threonine kinase that functions in numerous signaling pathways initiated by diverse stimuli. The functions of GSK-3 in cancer differ depending on cell type. In the present study, we examined the effects of a specific GSK-3 inhibitor on the regulation of osteosarcoma cell proliferation and apoptosis. Immunohistochemical analysis and real-time reverse transcription-polymerase chain reaction (RT-PCR) were performed to determine the expression pattern of GSK-3 in human osteosarcoma cells. We used the MTS assay, western blotting, measurement of single-stranded DNA and morphological analyses to study the effects of a GSK-3 inhibitor, SB216763 on osteosarcoma cell proliferation and survival. We detected an increase in mRNA expression of GSK-3 and aberrant nuclear accumulation of GSK-3 in the osteosarcoma cells. Pharmacological inhibition of GSK-3 led to a decrease in proliferation and survival of osteosarcoma cells. Inhibition of GSK-3 resulted in a decreased expression of Bcl-2 and a subsequent increase in osteosarcoma cell apoptosis via the mitochondrial pathway. The present study demonstrated that GSK-3 activity is critical for tumorigenicity and cell survival in osteosarcoma cells. Ou...Continue Reading

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Citations

Jun 7, 2020·Cells·Takahiro DomotoToshinari Minamoto
Jan 23, 2019·Frontiers in Pharmacology·Sorin HostiucEduard Drima
Jan 6, 2017·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Amy WalzAndrew P Mazar

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