GSK3 inhibitor-BIO regulates proliferation of female germline stem cells from the postnatal mouse ovary.

Cell Proliferation
Y HuJ Hua

Abstract

It is widely believed that in most female mammalian neonates, all germ cells enter meiosis to form the primary oocyte at the end of foetal development, and as a result, the postnatal mammalian ovary harbours only a limited supply of oocytes that cannot be regenerated. However, this idea has been challenged by the discovery of the existence of female germline stem cells (FGSCs) in postnatal mammalian ovaries. We have isolated ovarian GSCs from neonatal and adult mouse ovaries and expanded them in the same culture conditions as embryonic stem cells (ESCs). LIF and BIO were beneficial for formation of FGSC colonies. BIO promoted proliferation of FGSCs through activation of β-catenin and up-regulation of E-cadherin. The FGSCs formed compact round colonies with unclear borders, maintained ESC characteristics and alkaline phosphatase (AP) activity, expressing germ-cell markers-Vasa, and stem-cell markers: Oct4, Klf4, C-myc, Nanog, CD49f, Sox2, CD133, SSEA1 and SSEA4. These cells had the ability to form embryoid bodies (EBs), which expressed specific markers for all three germ layers. Then we induced EBs to differentiate into neurons, cardiomyocytes, pancreatic cells and germ cells, which showed the expression of specific markers, β-I...Continue Reading

References

Mar 8, 2000·Mechanisms of Development·S J ArnoldR Kemler
Jul 21, 2001·Stem Cells·M Pesce, H R Schöler
May 6, 2003·Science·Karin HübnerHans R Schöler
Feb 14, 2004·Human Molecular Genetics·Amander T ClarkRenee A Reijo Pera
Mar 12, 2004·Nature·Joshua JohnsonJonathan L Tilly
May 25, 2004·Nature Genetics·José A CostoyaPier Paolo Pandolfi
Sep 24, 2004·Current Opinion in Genetics & Development·Jianbo Wang, Anthony Wynshaw-Boris
Dec 17, 2004·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
Mar 15, 2006·Biochemical and Biophysical Research Communications·Kazuya OgawaHitoshi Niwa
Mar 28, 2006·Nature·Kaomei GuanGerd Hasenfuss
Mar 28, 2006·Nature Cell Biology·Paul W DyceJulang Li
May 9, 2006·Proceedings of the National Academy of Sciences of the United States of America·Mito Kanatsu-ShinoharaTakashi Shinohara
Jun 6, 2006·Genes & Development·Karl Willert, Katherine A Jones
Jun 24, 2006·The Journal of Biological Chemistry·Michael D Gordon, Roel Nusse
Jan 24, 2007·The Journal of Biological Chemistry·Rainer MussmannMatthias Austen
May 18, 2007·Development·Ting Xie, Linheng Li
May 23, 2007·Journal of Neuro-oncology·Rudolph J SchrotJames M Angelastro
Jul 20, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Masuko Katoh, Masaru Katoh
Sep 18, 2007·Stem Cell Reviews·Oliver Dreesen, Ali H Brivanlou
Nov 29, 2007·Molecular Cell·Nathalie RocquesCelio Pouponnot
Jan 18, 2008·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
May 3, 2008·Differentiation; Research in Biological Diversity·Irma Virant-KlunHelena Meden-Vrtovec
Oct 14, 2008·Nature·Sabine ConradThomas Skutella
Mar 14, 2009·Stem Cells and Development·Nady GolestanehMartin Dym
Jul 3, 2009·Cell Stem Cell·Kinarm KoHans R Schöler
Aug 19, 2009·Human Reproduction·Gulcin Abban, Joshua Johnson
Feb 9, 2010·Differentiation; Research in Biological Diversity·Jason PacchiarottiFariborz Izadyar
Mar 2, 2010·Fertility and Sterility·Seung Pyo GongJeong Mook Lim
Jul 14, 2010·Cell Stem Cell·Jose M Polo, Konrad Hochedlinger
Oct 26, 2010·Pharmacology & Therapeutics·Wojciech WojakowskiMichał Tendera
Nov 9, 2010·Stem Cells and Development·Paul W DyceJulang Li
Jun 30, 2011·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Antonin Bukovsky

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Citations

Sep 6, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Hua ZhangKui Liu
Aug 27, 2014·Stem Cell Research & Therapy·Cheryl E DunlopRichard A Anderson
Dec 21, 2013·Zygote : the Biology of Gametes and Early Embryos·Yashar EsmaeilianEsra Erdemli
May 23, 2013·Maturitas·Cheryl E DunlopRichard A Anderson
Apr 3, 2015·Biology of Reproduction·André Monteiro da RochaGary D Smith
Feb 14, 2018·International Journal of Molecular Sciences·Xiaobin HuangMarcel Karperien
Apr 5, 2017·Cellular Reprogramming·Hossein YazdekhastiMehdi Abbasi
Aug 7, 2017·Zygote : the Biology of Gametes and Early Embryos·Tania Janeth Porras-Gómez, Norma Moreno-Mendoza
Oct 22, 2016·Journal of Ovarian Research·Hossein YazdekhastiMehdi Abbasi

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