GSK3β negatively regulates HIF1α mRNA stability via nucleolin in the MG63 osteosarcoma cell line

Biochemical and Biophysical Research Communications
Dong-dong ChengQing-cheng Yang

Abstract

Hypoxia-inducible factor 1α (HIF1α) is a transcription factor involved in the growth, invasion and metastasis of malignant tumors. Glycogen synthase kinase 3 beta (GSK3β) is a protein kinase involved in a variety of signaling pathways, such as the Wnt and NF-κB pathways; this kinase can affect tumor progress through the regulation of transcription factor expression and apoptosis. Recent studies showed that GSK3β was involved in the expression of HIF1α. However, the effect of GSK3β on HIF1α expression in osteosarcoma cells remains unknown. To understand the relationship between GSK3β and HIF1α comprehensively, small RNA interference techniques, Western blot analyses, quantitative real-time PCR analyses and luciferase assays were used in our study. Experimental data revealed that inhibition of GSK3β could increase HIF1α protein levels and expression of its target genes by increasing the stability of the HIF1α mRNA, not by affecting the HIF1α protein stability, and that this process could be mediated by nucleolin.

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Citations

Jan 15, 2016·Biochemical and Biophysical Research Communications·Ronghai WangGuangjun Shi
Apr 18, 2015·Journal of Experimental & Clinical Cancer Research : CR·Tu HuQingcheng Yang
Jul 19, 2015·Biochemical and Biophysical Research Communications·Uk-Il JuYang-Sook Chun
Apr 14, 2016·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Zheng-Qi BaoDa-Min Chai

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