Abstract
Hypoxia-inducible factor 1α (HIF1α) is a transcription factor involved in the growth, invasion and metastasis of malignant tumors. Glycogen synthase kinase 3 beta (GSK3β) is a protein kinase involved in a variety of signaling pathways, such as the Wnt and NF-κB pathways; this kinase can affect tumor progress through the regulation of transcription factor expression and apoptosis. Recent studies showed that GSK3β was involved in the expression of HIF1α. However, the effect of GSK3β on HIF1α expression in osteosarcoma cells remains unknown. To understand the relationship between GSK3β and HIF1α comprehensively, small RNA interference techniques, Western blot analyses, quantitative real-time PCR analyses and luciferase assays were used in our study. Experimental data revealed that inhibition of GSK3β could increase HIF1α protein levels and expression of its target genes by increasing the stability of the HIF1α mRNA, not by affecting the HIF1α protein stability, and that this process could be mediated by nucleolin.
References
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·G L WangG L Semenza
Sep 5, 1997·The Journal of Biological Chemistry·S Salceda, J Caro
Oct 31, 1998·Current Opinion in Genetics & Development·G L Semenza
Jul 30, 1999·Biochemical and Biophysical Research Communications·E MinetC Michiels
Apr 9, 2001·Science·M IvanW G Kaelin
Dec 10, 2002·Science·G ManningS Sudarsanam
Mar 5, 2003·Journal of Cell Science·Bradley W Doble, James R Woodgett
Sep 18, 2004·Blood·Dominador J ManaloGregg L Semenza
Oct 11, 2005·Cell·Masatoshi TakagiMichael B Kastan
Oct 20, 2005·Science's STKE : Signal Transduction Knowledge Environment·Roland H WengerGieri Camenisch
May 24, 2006·Critical Reviews in Oncology/hematology·Kiichi Hirota, Gregg L Semenza
Jan 3, 2008·Cancer Cell·Tiebang KangHelen Piwnica-Worms
Jul 8, 2009·The Journal of Biological Chemistry·Verónica GonzálezDaekyu Sun
Jul 25, 2009·Oral Oncology·Anak Iamaroon, Suttichai Krisanaprakornkit
Jun 12, 2010·Molecular Cancer·Rajakishore Mishra
Dec 28, 2011·Carcinogenesis·Benoît MarchandMarie-Josée Boucher
Aug 23, 2012·The Journal of Biological Chemistry·Dongyun ZhangChuanshu Huang
Apr 6, 2013·Cell Death & Disease·E GavilánD Ruano
Jul 16, 2013·Oncogene·S-H KaoP-C Yang
Aug 15, 2013·International Journal of Molecular Sciences·Yan YinLiang Ma
Sep 11, 2013·The Biochemical Journal·I-Ju YehMonica M Montano
Citations
Apr 16, 2015·Biomolecules·Zhong-Jian Shen, James S Malter
Jan 15, 2016·Biochemical and Biophysical Research Communications·Ronghai WangGuangjun Shi
Apr 18, 2015·Journal of Experimental & Clinical Cancer Research : CR·Tu HuQingcheng Yang
Jul 19, 2015·Biochemical and Biophysical Research Communications·Uk-Il JuYang-Sook Chun
Apr 14, 2016·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Zheng-Qi BaoDa-Min Chai
Aug 16, 2016·PloS One·Lili CaoYe Zhang
Oct 27, 2016·Bulletin of Experimental Biology and Medicine·A V MorgunA B Salmina
Mar 8, 2018·Journal of Cellular and Molecular Medicine·Jiang ZouKangkai Wang
Dec 22, 2016·Oncotarget·James A McCubreyMassimo Libra
Dec 7, 2017·Oncotarget·Yan ZhangZhenggang Bi
Jul 29, 2020·Frontiers in Microbiology·Yange LiuDi Wang
Aug 25, 2018·Cancer Gene Therapy·Changbao ChenXinlong Ma