PMID: 2119502Sep 1, 1990Paper

GTP-binding proteins in rat liver nuclear envelopes

Proceedings of the National Academy of Sciences of the United States of America
J B RubinsN Riedel

Abstract

Nuclear transport as well as reassembly of the nuclear envelope (NE) after completion of mitosis are processes that have been shown to require GTP and ATP. To study the presence and localization of GTP-binding proteins in the NE, we have combined complementary techniques of [alpha-32P]GTP binding to Western-blotted proteins and UV crosslinking of [alpha-32P]GTP with well-established procedures for NE subfractionation. GTP binding to blotted NE proteins revealed five low molecular mass GTP-binding proteins of 26, 25, 24.5, 24, and 23 kDa, and [alpha-32P]GTP photoaffinity labeling revealed major proteins with apparent molecular masses of 140, 53, 47, 33, and 31 kDa. All GTP-binding proteins appear to localize preferentially to the inner nuclear membrane, possibly to the interface between inner nuclear membrane and lamina. Despite the evolutionary conservation between the NE and the rough endoplasmic reticulum, the GTP-binding proteins identified differed between these two compartments. Most notably, the 68- and 30-kDa GTP-binding subunits of the signal recognition particle receptor, which photolabeled with [alpha-32P]GTP in the rough endoplasmic reticulum fraction, were totally excluded from the NE fraction. Conversely, a major 5...Continue Reading

References

Jan 1, 1977·The Journal of Cell Biology·S E StuartR J Patterson
Feb 27, 1989·FEBS Letters·S TakedaK Sekimizu
Jun 23, 1989·Science·L S MayorgaP D Stahl
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J O BendittN Riedel
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·E G Lapetina, B R Reep
Sep 1, 1974·The Journal of Cell Biology·R P Aaronson, G Blobel
Oct 1, 1982·European Journal of Biochemistry·C Kondor-KochH Fischer
May 1, 1983·Experimental Cell Research·G G Maul, F Baglia
Dec 1, 1981·The Journal of Cell Biology·W W FrankeE D Jarasch
Nov 1, 1980·Experimental Cell Research·G G Maul, N Avdalović

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Citations

Dec 18, 2001·Journal of Cellular Biochemistry·P Phillips, M Thomson
Sep 1, 1996·Pflügers Archiv : European journal of physiology·G FolprechtH Oberleithner
Aug 10, 1992·Biochimica Et Biophysica Acta·K VerheydenG P Mannaerts
Jan 1, 1993·Advances in Enzyme Regulation·L CoccoF A Manzoli
Apr 27, 1995·Biochimica Et Biophysica Acta·M ThomsonP F Hall
Oct 1, 1992·Neurochemistry International·H OttoF Hucho
Dec 20, 1995·Biochimica Et Biophysica Acta·P CsermelyI Szántó
Jul 11, 2001·Prostaglandins & Other Lipid Mediators·B G MiguelA M Martínez
Aug 18, 2000·Immunology and Cell Biology·F S Willard, M F Crouch
Jan 1, 1992·The Journal of Cell Biology·A L BomanK L Wilson
Jun 1, 1996·The Journal of Cell Biology·D J Sweet, L Gerace
Nov 15, 1992·European Journal of Biochemistry·R BeckmannF Hucho
Mar 1, 1992·American Journal of Respiratory Cell and Molecular Biology·J B RubinsB F Dickey
Nov 6, 1992·Science·C Dingwall, R Laskey

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