GTP-cyclohydrolase-I like immunoreactivity in rat brain

Brain Research
D DassesseA Résibois

Abstract

GTPCH-I immunoreactive structures in the rat brain were studied using a polyclonal antibody raised in the chick. General mapping was made using the avidin-biotin-peroxidase technique and compared with the distribution of tyrosine hydroxylase and serotonin immunoreactivities. Double immunofluorescence was performed in order to establish real intracellular colocalization. GTPCH-I immunoreactivity was generally found to be low. Immunostained neurons were present in all the serotonin cell groups. In catecholaminergic neurons, although tyrosine hydroxylase immunoreactivity was always very high, GTPCH-I immunoreactivity was extremely variable, from relatively strong (substantia nigra, ventral tegmental area) to low (locus coeruleus, caudal part of the hypothalamus), extremely low (rostral hypothalamus, ventral brainstem) or almost absent (dorsal brainstem, some hypothalamic nuclei). When feasible, double immunolabeling revealed that all the serotonin cells and most of the tyrosine hydroxylase cells were also expressing GTPCH-I. Our results argue in favor of a regulation of tyrosine hydroxylase activity by the intracellular synthesis of BH4.

References

May 1, 1979·Journal of Neurochemistry·R A LevineW Lovenberg
Feb 15, 1978·The Journal of Comparative Neurology·R Y Moore
Jan 1, 1992·Neuroscience·S R Vincent, H Kimura
Dec 29, 1992·Biochimica Et Biophysica Acta·M GütlichI Ziegler
Aug 31, 1992·Biochemical and Biophysical Research Communications·A TogariT Nagatsu
Jan 2, 1989·European Journal of Biochemistry·G SchoedonH C Curtius
Jan 1, 1985·Annual Review of Biochemistry·C A NicholD S Duch
Jan 1, 1989·The Journal of Comparative Neurology·B W Newton, R W Hamill
Oct 1, 1987·Neuroscience·D WeissmannJ F Pujol
Mar 15, 1985·The Journal of Comparative Neurology·M Kalia, K Fuxe
Apr 1, 1974·The Journal of Comparative Neurology·O LindvallU Stenevi
Jan 1, 1981·Journal of Immunological Methods·J C JenseniusC Koch
Oct 1, 1982·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S M Hsu, E Soban
Jan 1, 1993·Advances in Experimental Medicine and Biology·I ZieglerL Hültner
Mar 1, 1994·Neuroscience·N J DunU Förstermann
Jan 1, 1995·Annual Review of Physiology·O W Griffith, D J Stuehr
Jan 1, 1993·Advances in Experimental Medicine and Biology·S I LentzG Kapatos
Jan 1, 1993·Advances in Experimental Medicine and Biology·K HirayamaG Kapatos
Jan 1, 1993·Advances in Experimental Medicine and Biology·M HoshigaH Kagamiyama
Mar 15, 1993·Biochemical and Biophysical Research Communications·T NomuraT Nagatsu
Jan 1, 1995·Journal of Neural Transmission. General Section·I NagatsuT Nagatsu
Dec 1, 1963·Proceedings of the National Academy of Sciences of the United States of America·S KAUFMAN

❮ Previous
Next ❯

Citations

Jan 20, 2004·Biochimica Et Biophysica Acta·Lina ElzaoukBeat Thöny
Jan 29, 2000·Brain Research. Molecular Brain Research·V DepaepeA Résibois
Mar 23, 2000·The Biochemical Journal·B ThönyN Blau
Feb 26, 2000·Acta Physiologica Scandinavica·T Flatmark
May 21, 2013·Cell Communication and Signaling : CCS·Johannes MeiserKarsten Hiller
Jan 19, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Vered HoldengreberYehuda Ben-Shaul
Sep 30, 2003·The Journal of Biological Chemistry·Mark GilchristA Dean Befus
May 16, 2009·Biochemical and Biophysical Research Communications·Jianhai DuYang Shi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.