GTP-dependent twisting of dynamin implicates constriction and tension in membrane fission

Nature
Aurélien RouxPietro De Camilli

Abstract

Dynamin, a crucial factor in endocytosis, is a member of a family of GTPases that participates in membrane fission. It was initially proposed to act as a machine that constricts and cuts the neck of nascent vesicles in a GTP-hydrolysis-dependent reaction, but subsequent studies suggested alternative models. Here we monitored the effect of nucleotides on dynamin-coated lipid tubules in real time. Addition of GTP, but not of GDP or GTP-gammaS, resulted in twisting of the tubules and supercoiling, suggesting a rotatory movement of the helix turns relative to each other during GTP hydrolysis. Rotation was confirmed by the movement of beads attached to the tubules. Twisting activity produced a longitudinal tension that was released by tubule breakage when both ends of the tubule were anchored. Fission also occurred when dynamin and GTP were added to lipid tubules that had been generated from liposomes by the motor activity of kinesin on microtubules. No fission events were observed in the absence of longitudinal tension. These findings demonstrate a mechanoenzyme activity of dynamin in endocytosis, but also imply that constriction is not sufficient for fission. At the short necks of endocytic vesicles, other factors leading to tensi...Continue Reading

References

Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·A MoschoR N Zare
Sep 7, 2000·The Journal of Cell Biology·B QualmannR B Kelly
Mar 22, 2001·Nature Reviews. Neuroscience·V I Slepnev, P De Camilli
Oct 5, 2001·Nature Cell Biology·P Zhang, J E Hinshaw
Oct 18, 2001·The Journal of Cell Biology·K FarsadP De Camilli
Nov 7, 2001·Current Biology : CB·H M Thompson, M A McNiven
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Aurélien RouxPatricia Bassereau
Jan 9, 2003·Current Opinion in Cell Biology·James D Orth, Mark A McNiven
Mar 4, 2003·Molecular and Cellular Biology·Hong CaoMark A McNiven
Aug 7, 2003·Proceedings of the National Academy of Sciences of the United States of America·G CharvinV Croquette
Oct 4, 2003·Physical Review Letters·Ilan TsafrirJoel Stavans
Mar 26, 2004·Nature Reviews. Molecular Cell Biology·Gerrit J K Praefcke, Harvey T McMahon
May 11, 2004·Nature Structural & Molecular Biology·Yen-Ju ChenJenny E Hinshaw
Jun 8, 2004·Traffic·Dorothy A Schafer
Sep 29, 2004·Journal of Structural Biology·Dganit DaninoJenny E Hinshaw
Dec 1, 2004·Proceedings of the National Academy of Sciences of the United States of America·Cécile LeducJacques Prost

❮ Previous
Next ❯

Citations

Jan 25, 2008·Histochemistry and Cell Biology·Alberto LuiniRoman S Polishchuk
Dec 11, 2007·Pflügers Archiv : European journal of physiology·Yumi Noda, Sei Sasaki
Feb 4, 2010·Cell Research·Sudha KumariSatyajit Mayor
May 6, 2010·Nature Cell Biology·Patricia Bassereau
Nov 17, 2009·Nature Cell Biology·Saumya PantBarth D Grant
Feb 8, 2011·Nature Cell Biology·Jeffrey B DoyonDavid G Drubin
Apr 24, 2012·Nature Cell Biology·Philip N Dannhauser, Ernst J Ungewickell
Dec 11, 2007·Nature Neuroscience·Jianhua XuLing-Gang Wu
Mar 21, 2008·Nature Reviews. Molecular Cell Biology·Maria Antonietta De Matteis, Alberto Luini
Aug 13, 2009·Nature Reviews. Molecular Cell Biology·Allen P Liu, Daniel A Fletcher
Aug 22, 2009·Nature Reviews. Molecular Cell Biology·Linton M Traub
Aug 22, 2009·Nature Reviews. Molecular Cell Biology·Barth D Grant, Julie G Donaldson
Nov 26, 2010·Nature Reviews. Molecular Cell Biology·Benedikt Westermann
Jul 23, 2011·Nature Reviews. Molecular Cell Biology·Harvey T McMahon, Emmanuel Boucrot
Jan 12, 2012·Nature Reviews. Molecular Cell Biology·Shawn M Ferguson, Pietro De Camilli
Dec 21, 2010·Nature Structural & Molecular Biology·Jason A MearsJenny E Hinshaw
Sep 2, 2008·Oncogene·B D'SouzaG Weinmaster
Jul 28, 2011·Integrative Biology : Quantitative Biosciences From Nano to Macro·Vyas RamananRavi Radhakrishnan
Jan 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Viviane PouponPeter S McPherson
Feb 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·Mitsuko HayashiPietro De Camilli
Aug 12, 2008·Proceedings of the National Academy of Sciences of the United States of America·Rainer BeckFelix Wieland
Nov 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jean-Baptiste MannevilleBruno Goud
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kelley A BethoneyMark A Lemmon
Feb 18, 2010·Proceedings of the National Academy of Sciences of the United States of America·Aurélien RouxPatricia Bassereau
May 20, 2011·Proceedings of the National Academy of Sciences of the United States of America·David L RichmondDaniel A Fletcher
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Xuelin LouPietro De Camilli
Jul 17, 2009·The Journal of Biological Chemistry·Olivia L MoorenDorothy A Schafer
Dec 2, 2009·The Journal of General Physiology·Fraser J MossHenry A Lester
Mar 6, 2009·Molecular Biology of the Cell·Mikhail V EgorovRoman S Polishchuk
May 28, 2010·Molecular Biology of the Cell·Miriam HammermeisterBenedikt Westermann
Apr 5, 2011·Molecular Biology of the Cell·Arun AnantharamRonald W Holz
Jul 23, 2008·American Journal of Therapeutics·Cynthia Y AbbanPatricio I Meneses
Mar 14, 2007·Current Opinion in Lipidology·Aaron Z Fernandis, Markus R Wenk
May 27, 2008·The Plant Cell·Catherine A KonopkaSebastian Y Bednarek

❮ Previous
Next ❯

Related Concepts

Related Feeds

BioHub - Researcher Network

The Chan-Zuckerberg Biohub aims to support the fundamental research and develop the technologies that will enable physicians to cure, prevent, or manage all diseases in our childrens' lifetimes. The CZ Biohub brings together researchers from UC Berkeley, Stanford, and UCSF. Find the latest research from the CZ Biohub researcher network here.