PMID: 2111192Feb 1, 1990Paper

GTP gamma S-induced calcium transients and exocytosis in human neutrophils

Bioscience Reports
O Nüsse, M Lindau

Abstract

Exocytosis and intracellular free calcium ([Ca2+]in) were simultaneously recorded in single human neutrophils using patch-clamp capacitance measurements and the fura-2 fluorescence ratio method. Intracellular application of guanosine-5'-O(3-thiotriphosphate) (GTP gamma S) stimulates both exocytosis and a calcium transient. The calcium transient starts to develop after a lag phase of approximately 40 s and normally appears to trigger the onset of exocytosis indicated by the beginning of the capacitance increase. After this delay [Ca2+]in increases from approximately 150 nM to approximately 600 nM with a sigmoidal time course. The peak concentration is reached within approximately 30 s but the main increase occurs during approximately 3 s. [Ca2+]in subsequently decays within 1-2 min to a level which is close to the resting value. This calcium transient is due to calcium release from inositoltrisphosphate-sensitive intracellular stores. Exocytosis also occurs if the calcium transient is abolished by intracellular EGTA but the lag phase is markedly prolonged. The GTP gamma S-induced calcium transient is very similar to that observed after stimulation with N-formyl-methionyl-leucyl-phenylalanine. The interplay between guanine nucleo...Continue Reading

References

Feb 1, 1988·Pflügers Archiv : European journal of physiology·M Pusch, E Neher
May 1, 1985·The Journal of Cell Biology·E L BeckerR I Sha'afi
Dec 1, 1988·The Journal of Cell Biology·O Nüsse, M Lindau
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R Penner
Feb 1, 1988·Pflügers Archiv : European journal of physiology·M Lindau, E Neher
Nov 12, 1987·Biochimica Et Biophysica Acta·D E Chandler, C J Kazilek

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Citations

Jan 5, 2000·Journal of Immunological Methods·K Lollike, M Lindau
Feb 1, 1991·Quarterly Reviews of Biophysics·M Lindau
Nov 10, 1998·The Journal of General Physiology·D LorenzM Bienert
Apr 12, 2017·Journal of Periodontology·Benjar Issaranggun Na AyuthayaPrasit Pavasant
Aug 2, 2001·The Journal of Biological Chemistry·A DiD J Nelson
May 3, 2018·Pflügers Archiv : European journal of physiology·Karolina NajderBenedikt Fels
Dec 12, 1991·Biochimica Et Biophysica Acta·M Lindau, B D Gomperts

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