GTP hydrolysis is essential for protein import into the mitochondrial matrix.

The Journal of Biological Chemistry
Naresh Babu V SepuriDebkumar Pain

Abstract

Protein import into the innermost compartment of mitochondria (the matrix) requires a membrane potential (delta psi) across the inner membrane, as well as ATP-dependent interactions with chaperones in the matrix and cytosol. The role of nucleoside triphosphates other than ATP during import into the matrix, however, remains to be determined. Import of urea-denatured precursors does not require cytosolic chaperones. We have therefore used a purified and urea-denatured preprotein in our import assays to bypass the requirement of external ATP. Using this modified system, we demonstrate that GTP stimulates protein import into the matrix; the stimulatory effect is directly mediated by GTP hydrolysis and does not result from conversion of GTP to ATP. Both external GTP and matrix ATP are necessary; neither one can substitute for the other if efficient import is to be achieved. These results suggest a "push-pull" mechanism of import, which may be common to other post-translational translocation pathways.

References

May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S M SimonG F Oster
Nov 2, 1990·Cell·W NeupertN Pfanner
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S T Hwang, G Schatz
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G Blobel
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·N G KronidouM Horst
Jan 1, 1994·Annual Review of Cell Biology·P Walter, A E Johnson
Oct 27, 1994·Nature·H C SchneiderW Neupert
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·H MurakamiD Pain
Mar 15, 1996·Science·D Görlich, I W Mattaj
Mar 15, 1996·Science·G Schatz, B Dobberstein
Apr 12, 1996·Science·J E Rothman, F T Wieland
Feb 1, 1997·Current Biology : CB·N Pfanner, M Meijer
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·N SchülkeD Pain
Oct 1, 1991·Trends in Cell Biology·B GlickG Schatz

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Citations

Nov 5, 1999·Biochimica Et Biophysica Acta·W VoosN Pfanner
Apr 25, 2013·Journal of the American Chemical Society·Simon A McManus, Yingfu Li
Apr 30, 2009·The Journal of Biological Chemistry·Hindupur K AnandatheerthavaradaNarayan G Avadhani
Dec 29, 2012·The Journal of Biological Chemistry·Prasad TammineniNaresh Babu Venkata Sepuri
May 16, 2008·The Journal of Biological Chemistry·Hindupur K AnandatheerthavaradaNarayan G Avadhani
Jan 25, 2013·Molecular Biology of the Cell·Adinarayana MaradaNaresh Babu V Sepuri
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·H S BoseW L Miller
Jul 11, 1998·The Journal of Biological Chemistry·S A KnightA Dancis
Apr 16, 2019·Chembiochem : a European Journal of Chemical Biology·Angela BergThorsten Berg
Jun 27, 1998·The Journal of Biological Chemistry·K S Crowley, R M Payne
Jul 18, 2006·The Biochemical Journal·Donna M GordonDebkumar Pain
Nov 22, 2007·The Journal of Biological Chemistry·Boominathan AmuthaDebkumar Pain
Nov 5, 2002·Cell Biochemistry and Function·Murray Thomson

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