Gts1p stabilizes oscillations in energy metabolism by activating the transcription of TPS1 encoding trehalose-6-phosphate synthase 1 in the yeast Saccharomyces cerevisiae

The Biochemical Journal
Zhaojun XuKunio Tsurugi

Abstract

We reported previously that Gts1p regulates oscillations of heat resistance in concert with those of energy metabolism in continuous cultures of the yeast Saccharomyces cerevisiae by inducing fluctuations in the levels of trehalose, but not in those of Hsp104 (heat shock protein 104). Further, the expression of TPS1, encoding trehalose-6-phosphate synthase 1, and HSP104 was activated by Gts1p in combination with Snf1 kinase, a transcriptional activator of glucose-repressible genes, in batch cultures under derepressed conditions. Here we show that, in continuous cultures, the mRNA level of TPS1 increased 6-fold in the early respiro-fermentative phase, while that of HSP104 did not change. The expression of SUC2, a representative glucose-repressible gene encoding invertase, also fluctuated, suggesting the involvement of the Snf1 kinase in the periodic activation of these genes. However, this possibility was proven to be unlikely, since the oscillations in both TPS1 and SUC2 mRNA expression were reduced by approx. 3-fold during the transient oscillation in gts1Delta (GTS1-deleted) cells, in which the energy state determined by extracellular glucose and intracellular adenine nucleotide levels was comparable with that in wild-type ce...Continue Reading

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Citations

Nov 11, 2006·Annual Review of Genetics·Herman Wijnen, Michael W Young
Feb 11, 2011·Bioscience, Biotechnology, and Biochemistry·Mitsuaki SanadaMitsuyoshi Ueda
Mar 10, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Xinyun LiKui Li
Mar 9, 2005·The FEBS Journal·Matthieu JulesJean Luc Parrou
Oct 11, 2014·PloS One·Ildefonso M De la FuenteLuis Martínez
May 4, 2005·Yeast
May 29, 2007·Archives of Biochemistry and Biophysics·Zhaojun Xu, Kunio Tsurugi

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