PMID: 2508627Sep 1, 1989Paper

Guanine-nucleotide-binding proteins expressed in rat white adipose tissue. Identification of both mRNAs and proteins corresponding to Gi1, Gi2 and Gi3

The Biochemical Journal
F M MitchellG Milligan

Abstract

Considerable debate has focused on the molecular identity of the guanine-nucleotide-binding proteins (G-proteins) in adipose tissue which can be detected following pertussis-toxin-catalysed ADP-ribosylation [Rapiejko, Northup, Evans, Brown & Malbon (1986) Biochem. J. 240, 35-40; Hinsch, Rosenthal, Spicher, Binder, Gausepohl, Frank, Schultz & Joost (1988) FEBS Lett. 238, 191-196]. We have used a panel of selective anti-peptide antisera which are able to discriminate between the different pertussis-toxin-sensitive G-proteins to assess which of these are expressed in rat adipose tissue. We demonstrate that plasma membranes of rat white adipocytes contain alpha subunits corresponding to each of Gi1, Gi2 and Gi3. Furthermore, using synthetic oligonucleotides complimentary to unique regions of each of the three polypeptides, we demonstrate that the mRNAs for the three G-protein alpha subunits can also be detected in adipose tissue.

Citations

Jul 18, 2002·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·S Y Nam, P E Lobie
Jan 1, 1993·Journal of Neurochemistry·R J Summers, L R McMartin
Jul 1, 1991·Acta Physiologica Scandinavica·H L VikmanJ J Ohisalo
Feb 19, 2002·Brain Research. Developmental Brain Research·Ivanna IhnatovychPetr Svoboda
Oct 6, 1997·Cellular Signalling·K DibC Correze
Jul 1, 1995·Brain Research. Molecular Brain Research·F C AbogadieA I Farbman
Mar 1, 1994·Molecular and Cellular Endocrinology·M CormontY Le Marchand-Brustel

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