PMID: 2112463May 20, 1990Paper

Guanine nucleotides and Ca2(+)-dependent lysosomal secretion in electropermeabilised human platelets

European Journal of Biochemistry
C M Athayde, M C Scrutton

Abstract

1. Metabolically stable analogues of GTP, e.g. guanosine 5'-[gamma-thio]triphosphate (GTP[S]) and guanosine 5'-[beta,gamma-imido]triphosphate (pp[NH]pG), enhance the extent of Ca2(+)-dependent secretion of beta-N-acetylglucosaminidase and beta-galactosidase from electropermeabilised human platelets in the presence of less than 5 microM Ca2+. A similar effect is observed on addition either of 1,2-dioctanoin or of GTP in in the presence or absence of thrombin. 2. In the presence of higher Ca2+ concentrations the extent of enhancement of lysosomal secretion declines and little, or no, enhancement is observed at a [Ca2+] of 30-40 microM. Addition of leupeptin or antipain prevents this decrease in lysosomal secretion and enhances the extent of Ca2(+)-dependent lysosomal secretion obtained in the presence or absence of guanine nucleotides, thrombin or 1,2-dioctanoin. 3. The concentration of GTP[S] or pp[NH]pG required to obtain half-maximal enhancement of lysosomal secretion is dependent on [Ca2+] for secretion of 5-hydroxytryptamine, beta-N-acetylglucosaminidase and beta-galactosidase. At two fixed [Ca2+] the median effective concentration (EC50) values for GTP[S] and pp[NH]pG which characterise enhancement of 5-hydroxytryptamine se...Continue Reading

References

Feb 1, 1989·Bioscience Reports·P E Tatham, B D Gomperts
Aug 1, 1984·Archives of Biochemistry and Biophysics·G N Memartino, D E Croall
May 1, 1980·Analytical Biochemistry·C A Dangelmaier, H Holmsen
May 29, 1981·Biochemical and Biophysical Research Communications·J A Truglia, A Stracher
May 1, 1964·The Journal of Clinical Investigation·R H ASTER, J H JANDL

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