Guanosine tetraphosphate (ppGpp)-mediated inhibition of the activity of the bacteriophage lambda pR promoter in Escherichia coli

Molecular & General Genetics : MGG
B WróbelG Wegrzyn

Abstract

It was previously demonstrated that the activity of bacteriophage lambda promoter pR is decreased in wild-type Escherichia coli cells starved for amino acids (during the stringent response). Since pR activity is necessary for the transcriptional activation of ori lambda, this leads to inhibition of the replication of plasmids derived from phage lambda. These results led to the proposal that the pR promoter susceptible to control by the stringent response. However, subsequent studies demonstrated that this promoter is activated by the host dnaA gene product and since the dnaA promoter was reported to be controlled by the stringent response, it is possible that the inhibition of pR activity in amino acid-starved cells is indirect, and results from the impairment of DnaA-mediated transcriptional activation. Here we present evidence that pR is negatively regulated by ppGpp, even when DnaA protein is provided in excess as well as in cells devoid of DnaA function. We have checked that the level of ppGpp is increased during prolonged (up to 4 h) starvation for isoleucine in relA+ cells but not in the relA- mutant. At the same time we observed inhibition of lambda plasmid replication during the stringent, but not relaxed, response, eve...Continue Reading

Citations

Feb 28, 2002·Nucleic Acids Research·Sylwia BarańskaGrzegorz Wegrzyn
Jan 31, 2007·Proceedings of the National Academy of Sciences of the United States of America·Li LiArthur Kornberg
Jun 22, 2002·Microbial Cell Factories·Grzegorz Wegrzyn, Alicja Wegrzyn
Sep 13, 2002·The Journal of Biological Chemistry·Katarzyna PotrykusV James Hernandez
Sep 3, 2013·Journal of Bacteriology·Dariusz NowickiAgnieszka Szalewska-Pałasz

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