Guanylate-Binding Protein 1, an Interferon-Induced GTPase, Exerts an Antiviral Activity against Classical Swine Fever Virus Depending on Its GTPase Activity

Journal of Virology
Lian-Feng LiHua-Ji Qiu

Abstract

Many viruses trigger the type I interferon (IFN) pathway upon infection, resulting in the transcription of hundreds of interferon-stimulated genes (ISGs), which define the antiviral state of the host. Classical swine fever virus (CSFV) is the causative agent of classical swine fever (CSF), a highly contagious viral disease endangering the pig industry in many countries. However, anti-CSFV ISGs are poorly documented. Here we screened 20 ISGs that are commonly induced by type I IFNs against CSFV in lentivirus-delivered cell lines, resulting in the identification of guanylate-binding protein 1 (GBP1) as a potent anti-CSFV ISG. We observed that overexpression of GBP1, an IFN-induced GTPase, remarkably suppressed CSFV replication, whereas knockdown of endogenous GBP1 expression by small interfering RNAs significantly promoted CSFV growth. Furthermore, we demonstrated that GBP1 acted mainly on the early phase of CSFV replication and inhibited the translation efficiency of the internal ribosome entry site of CSFV. In addition, we found that GBP1 was upregulated at the transcriptional level in CSFV-infected PK-15 cells and in various organs of CSFV-infected pigs. Coimmunoprecipitation and glutathione S-transferase (GST) pulldown assays...Continue Reading

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Citations

May 29, 2020·Journal of Virology·Xinyu ZhuShaobo Xiao
Sep 9, 2016·World Journal of Gastroenterology : WJG·Nathalie Britzen-LaurentMichael Stürzl
Jun 26, 2020·Frontiers in Microbiology·Yuming ChenJinding Chen
Dec 14, 2018·PLoS Pathogens·Zicong XieHongsheng Ouyang
Jun 28, 2019·Probiotics and Antimicrobial Proteins·Xiaoxiao GuDongmei Zhang
Jan 31, 2020·Frontiers in Oncology·Jing ZhaoZhenhe Suo
Dec 15, 2020·The FEBS Journal·Miriam Kutsch, Jörn Coers
Jan 20, 2020·Biochimica Et Biophysica Acta. Proteins and Proteomics·Sudeepa Rajan, Apurba Kumar Sau
Mar 7, 2021·Journal of Biomedical Science·Rongzhao ZhangChunfu Zheng
May 1, 2021·Microorganisms·Jindai FanJinding Chen
May 11, 2021·Frontiers in Cell and Developmental Biology·Mounira K Chelbi-Alix, Pierre Thibault
Nov 5, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tianle GuYong-Gang Yao

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Methods Mentioned

BETA
GTPases
GTPase
transfection
reverse transcription-PCR
pulldown
ELIPA
enzyme-linked
co-IP

Software Mentioned

SPSS
CoLocalizer Pro
ELIPA
Odyssey

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